Measurement of changes in the oxygenation of quadriceps muscles during the voluntary and involuntary fatigue test in normal healthy sedentary subjects


  • Amir Ur Rehman Denham Unit, Harrow and Ealing PCT NHS Trust, Harrow, Middlesex, United Kingdom
  • Muhammad A. Siddiqui Queen Margaret University, Edinburgh, United Kingdom
  • Haider Darain Khyber Medical University, Peshawar Queen Margaret University, Edinburgh, Pakistan



Muscle oxygenation, Muscle fatigue, Electrical stimulation, Cycle ergometry, Near infrared spectroscopy


The Purpose of this study is to investigate the changes in muscle oxygen consumption in response to the different fatigue protocol cycle ergometry and electrical stimulation (voluntary and involuntary) in human quadriceps muscle using near infrared spectroscopy (NIRS).Fifteen healthy sedentary voluntary University students between ages 20-60 were invited to participate in the study. Three minutes stimulation was performed to fatigue the muscle. Changes in muscle oxygenation were measured by near infrared spectroscopy. The present resistance was calculated as the estimated maximal power output. The data were analysed using the Kolmogorov-Smirnov (K-S) test to determine the distribution. Descriptive statistics are used to characterize the shape, central tendency, and variability within a set of data. Differences were tested by utilizing the Friedman test the level of statistical significance was set at P<0.05. There was no significant difference (p>0.05) was found between right leg oxygenated (∆HbO2), deoxygenated (∆HHb), and total haemoglobin (∆CHb) as compared to left leg during cycle ergometry fatigue test. On the other hand, significant difference (p<0.05) was found in oxygenated haemoglobin of right leg when two (cycle ergometry and electrical stimulation) fatigue results were compared. However, no significant difference (p>0.05) was found in deoxygenated (∆HHb) and total haemoglobin (∆CHb) of right leg when two (cycle ergometry and electrical stimulation) fatigue results were compared. There was no significant difference (p>0.05) found in oxygenated, deoxygenated and total haemoglobin between right and left leg cycle ergometry fatigue indices. Similarly, no significant difference (p>0.05) was found in oxygenated, deoxygenated and total haemoglobin of right leg when two (cycle ergometry and electrical stimulation) fatigue indices were compared. The significant difference (p<0.001) were found between two (cycle ergometry and electrical stimulation) fatigue results. This study reveals that the oxygen consumption was more in the electrical stimulation as compared to the cycle ergometry during the fatigue test. Significant difference was observed between the oxygenated haemoglobin when comparing the electrical stimulation with cycle ergometry. Similarly, significant differences were found between the legs in cycle ergometry fatigue test.


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How to Cite

Rehman, A. U., Siddiqui, M. A., & Darain, H. (2016). Measurement of changes in the oxygenation of quadriceps muscles during the voluntary and involuntary fatigue test in normal healthy sedentary subjects. Journal of Human Sport and Exercise, 10(4), 867–882.



Sport Medicine, Nutrition & Health