The effect of aerobic and anaerobic training with melatonin consumption on the expression of apoptotic genes BAX and BCL2 myocardial in rats after ischemic reperfusion


  • Hamed Alizadeh Pahlavani Behbahan Faculty of Medical Sciences, Islamic Azad University of Shooshtar Branch, Iran, Islamic Republic of
  • Ali Veisi Behbahan Faculty of Medical Sciences, Islamic Azad University of Shooshtar Branch, Iran, Islamic Republic of



Aerobic training, Anaerobic training, Melatonin, BAX, BCL-2


The present research aims to examine The Effect of aerobic and anaerobic training with melatonin consumption on the expression of apoptotic genes BAX and BCL2 myocardial in rats after ischemic reperfusion. In this study, Wistar rats (n = 38) were used weighing approximately 200-250 g with two to three months old. The study was conducted using Stroke (n = 7) and healthy (n = 7) rats to determine the stroke of isoprenaline injection; and left ventricular stroke was confirmed after the isoprenaline injection with the Terry Chromasone technique. Then, the rats were divided into 6 groups of aerobic (n = 4), anaerobic (n = 4), aerobic with melatonin (n = 4), anaerobic with melatonin (n = 4), Melatonin (n = 4) and control group (n = 4). The research group rats were familiar running on treadmill for a week, and One-month aerobic and anaerobic training was performed with three sessions per week. Also, melatonin gavage (10 mg / kg) was applied to melatonin treatment groups. After two rest days, all rats were injected with isoprenaline at 24 hours intervals. However, control group rats were only injected with isoprenaline. Data was analyzed using independent T, one way ANOVA and 2-∆∆ct formula. The results of this study showed that aerobic and anaerobic training and melatonin alone had a negligible effect on BAX and BCL2 genes expression,However, aerobic and anaerobic training combined with melatonin was able to reduce and increase the expression of the BAX and BCL2 genes, respectively. Of course, these changes had a significant effect on melatonin alone. Therefore, it seems that this intensity and duration of training with melatonin intake into melatonin alone can provide the anti-apoptotic routes of training to minimize the effects of acute stroke.


Download data is not yet available.


F, L., K. h, and L. j, Harrison's Principles of Internal Medicine. New York, NY: McGraw-Hill Professional, 2012. 18th ed.

M, S., Apoptosis programmed cell death. Arak, arak Univ Press, 2009. 1th ed.

Santana, E.T., et al., Aerobic exercise training induces an anti-apoptotic milieu in myocardial tissue. Motriz: Revista de Educação Física, 2014. 20: p. 233-238.

Kwak, H.-B., W. Song, and J.M. Lawler, Exercise training attenuates age-induced elevation in Bax/Bcl-2 ratio, apoptosis, and remodeling in the rat heart. The FASEB Journal, 2006. 20(6): p. 791-793.

Delchev, S., et al., Bcl-2/Bax ratio, mitochondrial membranes and aerobic enzyme activity in cardiomyocytes of rats after submaximal training. Vol. 48. 2006. 50-6.

Lajoie, C., A. Calderone, and L. Béliveau, Exercise training enhanced the expression of myocardial proteins related to cell protection in spontaneously hypertensive rats. Pflügers Archiv, 2004. 449(1): p. 26-32.

Jafari, A., et al., Effect of Exercise Training on Bcl-2 and Bax Gene Expression in the Rat Heart. Gene Cell Tissue, 2015.

Marsh, S.A., et al., Bcl-2 in endothelial cells is increased by vitamin E and α-lipoic acid supplementation but not exercise training. Journal of Molecular and Cellular Cardiology, 2005. 38(3): p. 445-451.

Sagiv, M., Exercise cardiopulmonary function in cardiac patients. Springer-Verlag London, 2012.

Pescatello, L.S., ACSM's guidelines for exercise testing and prescription. Wolters Kluwer/Lippincott Williams & Wilkins Health, 2014.

Farhud, D. and L. Yazdanpanah, Glucose-6-phosphate dehydrogenase (G6PD) Deficiency. Iranian journal of public health, 2008. 37(4): p. 1-18.

Petrosillo, G., et al., Protective effect of melatonin against mitochondrial dysfunction associated with cardiac ischemia-reperfusion: role of cardiolipin. The FASEB journal, 2006. 20(2): p. 269-276.

Patel, V., et al., Cardioprotective effect of melatonin against isoproterenol induced myocardial infarction in rats: a biochemical, electrocardiographic and histoarchitectural evaluation. European journal of pharmacology, 2010. 644(1): p. 160-168.

Forman, K., et al., Beneficial effects of melatonin on cardiological alterations in a murine model of accelerated aging. Journal of pineal research, 2010. 49(3): p. 312-320.

Azamianjazi, A., et al., The Combined Effect of Endurance Training and Atorvastatin on the Extent of Necrosis Damageand Fibrosis Tissue in Male Wistar Rats Heart after Experimental Myocardial Infarction. medilam, 2016. 23(7): p. 28-38.

Høydal, M.A., et al., Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. European Journal of Cardiovascular Prevention & Rehabilitation, 2007. 14(6): p. 753-760.

Vibha, L., et al., Protective Effect of Medicinal Garlic Against Isoprenaline Induced Myocardial Infarction in Rats. International Journal of Pharmacology, 2011. 7: p. 510-515.

Shahbaz, A.U., et al., Calcium and zinc dyshomeostasis during isoproterenol-induced acute stressor state. Am J Physiol Heart Circ Physiol, 2011. 300(2): p. H636-44.

Jin Ha, S. and W. Weon Kim, Mechanism of Ischemia and Reperfusion Injury to the Heart:From the Viewpoint of Nitric Oxide and Mitochondria. Chonnam Medical JournaL, 2010. 46 (3): p. 129-139.

Cooke, M.S., et al., Oxidative DNA damage: mechanisms, mutation, and disease. Faseb j, 2003. 17(10): p. 1195-214.

Guizoni, D.M., et al., Effects of late exercise on cardiac remodeling and myocardial calcium handling proteins in rats with moderate and large size myocardial infarction. Int J Cardiol, 2016. 221: p. 406-12.

de Sousa, C.V., et al., The Antioxidant Effect of Exercise: A Systematic Review and Meta-Analysis. Sports Med, 2017. 47(2): p. 277-293.

Luchetti, F., et al., Melatonin signaling and cell protection function. The FASEB Journal, 2010. 24(10): p. 3603-3624.

Heidari, R. and V. Nejati, Protective Effects of Melatonin on Lipid Profile in Fructose Induced Dyslipidemia. Iranian Journal of Endocrinology and Metabolism, 2011. 13(4): p. 406-411.

Hardeland, R., Atioxidative protection by melatonin. Endocrine, 2005. 27(2): p. 119-130.

Kang, P.M., et al., Alterations in apoptosis regulatory factors during hypertrophy and heart failure. Am J Physiol Heart Circ Physiol, 2004. 287(1): p. H72-80.

Marfe, G., et al., The modulation of sirtuins and apoptotic proteins in rats after exhaustive exercise. Open Journal of Molecular and Integrative Physiology, 2012. 2: p. 65-74.

Chengji, W. and H. Shoujun, Aerobic exercise can ameliorate heart function in patients with myocardial infarction through up-regulating M3 receptor. IJC Metabolic & Endocrine, 2016. 13(Supplement C): p. 1-5.

Powers, S.K., J.C. Quindry, and A.N. Kavazis, Exercise-induced cardioprotection against myocardial ischemia–reperfusion injury. Free Radical Biology and Medicine, 2008. 44(2): p. 193-201.

Veneroso, C., et al., Melatonin reduces cardiac inflammatory injury induced by acute exercise. Journal of pineal research, 2009. 47(2): p. 184-191.

Kumar, K.V. and M. Naidu, Effect of oral melatonin on exercise-induced oxidant stress in healthy subjects. Indian Journal of Pharmacology, 2002. 34(4): p. 256-259.

dos Santos, G.B., et al., Melatonin reduces oxidative stress and cardiovascular changes induced by stanozolol in rats exposed to swimming exercise. The Eurasian journal of medicine, 2013. 45(3): p. 155.



Statistics RUA



How to Cite

Alizadeh Pahlavani, H., & Veisi, A. (2018). The effect of aerobic and anaerobic training with melatonin consumption on the expression of apoptotic genes BAX and BCL2 myocardial in rats after ischemic reperfusion. Journal of Human Sport and Exercise, 13(2proc), S454-S466.