Salivary Immunoglobulin A responses to 6-minute walk test in elderly women
Keywords:6 Minute Walk Test, Blood lactate, Elderly women, Secretory immunoglobulin A
Objetive: The aim of the present study was evaluate the effect of the 6-minute walk test (6MWT) on the secretory immunoglobulin A (sIgA) kinetics in whole saliva of healthy elderly women. Methods: Nine healthy, elderly females (age 61 ± 6.7 years) performed the 6 MWT. Saliva samples and heart rate (HR) were measured in basal, immediately (IPE), and 30 minutes after the 6 MWT (30-Post Ex). Blood lactate [La] was measured at the same intervals. The ELISA assay was used to determine the sIgA concentrations [sIgA]. The Biuret assay was employed to determine the total proteins levels in saliva. Results: The exercise intensity reached by the participants was ≈ 84 + 16% of maximum HR. Baseline [La] was 1.8 ± 0.9 mmol/L. [La] at IPE increased 3.4 mmol/L above baseline (p = 0.02). [sIgA] increased by 233.3 + 109.3 % at IPE and remained 211.2 + 100.1 % greater at 30-Post Ex compared with basal (p = 0.02). The 6 MWT did not modify total proteins levels (p > 0.05). Conclusion: The 6 MWT, performed to submaximal intensity, appears to be enough of a stimulus to increases sIgA levels in healthy elderly women independent of total proteins concentration in saliva.
Akimoto, T., Kumai, Y., Akama, T., Hayashi, E., Murakami, H., Soma, R., … Kono, I. (2003). Effects of 12 months of exercise training on salivary secretory IgA levels in elderly subjects. British Journal of Sports Medicine, 37(February 2009), 76–79. https://doi.org/10.1136/bjsm.37.1.76
Allgrove, J. E., Gomes, E., Hough, J., & Gleeson, M. (2008). Effects of exercise intensity on salivary antimicrobial proteins and markers of stress in active men. Journal of Sports Sciences, 26(6), 653–661. https://doi.org/10.1080/02640410701716790
Astrand, P., & Ryhming, I. (1954). A nomogram for calculation of aerobic capacity (physical fitness) from pulse rate during sub-maximal work. J Appl Physiol, 7(2), 218–221. https://doi.org/10.1152/jappl.19126.96.36.199
Baralic, I., Miletic, I., Djordjevic, B., Terzic, T., Radojevic-Skodric, S., & Nikolic, G. (2010). Effect of sensory stimulation on salivary IgA secretion rate in karate players. Biology of Sport, 27(4), 273–278. https://doi.org/10.5604/20831862.927493
Bazzano, C., Cunningham, L. N., Cama, G., & Falconio, T. (1998). The relationship of lactate to 1-Mile walk performance in women aged 60 to 70 years. Journal of Aging & Physical Activity, 6, 285–289. https://doi.org/10.1123/japa.6.3.285
Bishop, N. C., Walker, G. J., Scanlon, G. A., Richards, S., & Rogers, E. (2006). Salivary IgA responses to prolonged intensive exercise following caffeine ingestion. Medicine and Science in Sports and Exercise, 38(3), 513–519. https://doi.org/10.1249/01.mss.0000187412.47477.ee
Carmona, J. J., & Michan, S. (2016). Biology of Healthy Aging and Longevity. Revista de Investigacion Clinica; Organo Del Hospital de Enfermedades de La Nutricion, 68(1), 7–16.
Chang, Y.-K., Pan, C.-Y., Chen, F.-T., Tsai, C.-L., & Huang, C.-C. (2012). Effect of Resistance-Exercise Training on Cognitive Function in Healthy Older Adults: A Review. Journal of Aging and Physical Activity, 20(4), 497–517. https://doi.org/10.1123/japa.20.4.497
Corthésy, B. (2013). Role of secretory IgA in infection and maintenance of homeostasis. Autoimmunity Reviews, 12(6), 661–665. https://doi.org/10.1016/j.autrev.2012.10.012
de Souza, J. D., Ribeiro, A. Q., Martinho, K. O., Franco, F. S., Martins, M. V, Rodrigues, M. G., … Tinôco, A. L. A. (2015). Lipid profile and associated factors among elderly people, attended at the Family Health Strategy, Viçosa/MG . Nutricion Hospitalaria, 32(2), 771–778. https://doi.org/10.3305/nh.2015.32.2.8875
Gleeson, M., & Pyne, D. B. (2015). Respiratory inflammation and infections in high-performance athletes. Immunology and Cell Biology, 94(2), 124–131. https://doi.org/10.1038/icb.2015.100
Guimarães, G. V., Bellotti, G., Bacal, F., Mocelin, A., & Bocchi, E. A. (2002). Can the cardiopulmonary 6-minute walk test reproduce the usual activities of patients with heart failure? Arquivos Brasileiros de Cardiologia, 78(6), 553–560. https://doi.org/10.1590/S0066-782X2002000600003
Guyatt, G. H., Sullivan, M. J., Thompson, P. J., Fallen, E. L., Pugsley, S. O., Taylor, D. W., & Berman, L. B. (1985). The 6-minute walk: A new measure of exercise capacity in patients with chronic heart failure. Can Med Assoc, 132(9), 919–923. https://doi.org/10.1016/j.physio.2011.11.003
Howel, D. (2012). Waist Circumference and Abdominal Obesity among Older Adults: Patterns, Prevalence and Trends. PLoS ONE, 7(10). https://doi.org/10.1371/journal.pone.0048528
Hwang, Y., Park, J., & Lim, K. (2016). Effects of pilates exercise on salivary Secretory immunoglobulin a levels in older women. Journal of Aging and Physical Activity, 24(3), 399–406. https://doi.org/10.1123/japa.2015-0005
Ihalainen, J. K., Schumann, M., Mero, A. A., & Ihalainen, J. K. (2016). Mucosal immunity and upper respiratory tract symptoms in recreational endurance runners. Physiology, Applied, 41(1), 96–102.
Janaudis-Ferreira, T., Sundelin, G., & Wadell, K. (2010). Comparison of the 6-minute walk distance test performed on a non-motorised treadmill and in a corridor in healthy elderly subjects. Physiotherapy, 96(3), 234–239. https://doi.org/10.1016/j.physio.2009.11.015
Jenkins, S., Cecins, N., Camarri, B., Williams, C., Thompson, P., & Eastwood, P. (2009). Regression equations to predict 6-minute walk distance in middle-aged and elderly adults. Physiotherapy Theory and Practice, 25(7), 516–522. https://doi.org/10.3109/09593980802664711
Li, T.-L., & Rush, B. (2009). The Effects of Prolonged Strenuous Exercise on Salivary Secretion of IgA Subclasses in Men. International Journal, 1(3), 69–74.
López-Otín, C., Blasco, M. A., Partridge, L., Serrano, M., & Kroemer, G. (2013). The hallmarks of aging. Cell, 153(6). https://doi.org/10.1016/j.cell.2013.05.039
Negreiros, A., Padula, R. S., Andrea Bretas Bernardes, R., de Moraes, M. V., Pires, R. S., & Chiavegato, L. D. (2017). Predictive validity analysis of six reference equations for the 6-minute walk test in healthy Brazilian men: a cross-sectional study. Brazilian Journal of Physical Therapy, 21(5), 350–356. https://doi.org/10.1016/j.bjpt.2017.06.003
Neville, V., Gleeson, M., & Folland, J. P. (2008). Salivary IgA as a risk factor for upper respiratory infections in elite professional athletes. Medicine and Science in Sports and Exercise, 40(7), 1228–1236. https://doi.org/10.1249/MSS.0b013e31816be9c3
Nieman, D. C. (1994). Exercise, upper respiratory tract infections and the immune system. Medicine Science Sport Exercise, 26 (2), 128-139. https://doi.org/10.1249/00005768-199402000-00002
Prcina, M., Novak, M., Cigankova, V., & Kontsekova, E. (2018). Immunosenescence - the role in the immunotherapy of older population. Bratislavské Lekárske Listy, 119(4), 217–220. https://doi.org/10.4149/BLL_2018_040
Rikli, R. E., & Jones, C. J. (1998). The Reliability and Validity of a 6-Minute Walk Test as a Measure of Physical Endurance in Older Adults. Journal of Aging & Physical Activity. https://doi.org/10.1097/00005768-199805001-00421
Sakamoto, Y., Ueki, S., Kasai, T., Takato, J., Shimanuki, H., Honda, H., … Haga, H. (2009). Effect of exercise, aging and functional capacity on acute secretory immunoglobulin A response in elderly people over 75 years of age. Geriatrics and Gerontology International, 9(1), 81–88. https://doi.org/10.1111/j.1447-0594.2008.00502.x
Sakamoto, Y., Ueki, S., Shimanuki, H., Kasai, T., Takato, J., Ozaki, H., … Haga, H. (2005). Effects of low-intensity physical exercise on acute changes in resting saliva secretory IgA levels in the elderly. Geriatrics and Gerontology International, 5(3), 202–206. https://doi.org/10.1111/j.1447-0594.2005.00297.x
Sari-Sarraf, V., Reilly, T., Doran, D. A., & Atkinson, G. (2007). The effects of single and repeated bouts of soccer-specific exercise on salivary IgA. Archives of Oral Biology, 52(6), 526–532. https://doi.org/10.1016/j.archoralbio.2006.11.016
Shanmugasundaram, M., Rough, S. J., & Alpert, J. S. (2010). Dyslipidemia in the elderly: Should it be treated? Clinical Cardiology, 33(1), 4–9. https://doi.org/10.1002/clc.20702
Shimizu, K., Kimura, F., Akimoto, T., Akama, T., Kuno, S., & Kono, I. (2007). Effect of free-living daily physical activity on salivary secretory IgA in elderly. Medicine and Science in Sports and Exercise, 39(4), 593–598. https://doi.org/10.1249/mss.0b013e318031306d
Sloan, C. A., Engels, H. J., Fahlman, M. M., Yarandi, H. E., & Davis, J. E. (2013). Effects of exercise on S-IGA and URS in postmenopausal women. International Journal of Sports Medicine, 34(1), 81–86. https://doi.org/10.1055/s-0032-1314817
Someya, F., Mugii, N., & Oohata, S. (2015). Cardiac hemodynamic response to the 6-minute walk test in young adults and the elderly. BMC Res Notes, 8, 355. https://doi.org/10.1186/s13104-015-1331-5
Teixeira, A. M., Martins, R., Martins, M., & Cunha, M. D. R. (2008). Changes in Functional Fitness, Mood States and Salivary IgA Levels after Exercise Training for 19 Weeks in Elderly Subjects. International Journal of Applied Sports Sciences, 20(2), 16–26.
Troosters, T., Vilaro, J., Rabinovich, R., Casas, A., Barberà, J. A., Rodriguez-Roisin, R., & Roca, J. (2002). Physiological responses to the 6-min walk test in patients with chronic obstructive pulmonary disease. European Respiratory Journal, 20(3), 564–569. https://doi.org/10.1183/09031936.02.02092001
Uemura, K., Doi, T., Shimada, H., Makizako, H., Yoshida, D., Tsutsumimoto, K., … Suzuki, T. (2012). Effects of Exercise Intervention on Vascular Risk Factors in Older Adults with Mild Cognitive Impairment: A Randomized Controlled Trial. Dementia and Geriatric Cognitive Disorders Extra, 2(1), 445–455. https://doi.org/10.1159/000343486
Usui, T., Yoshikawa, T., Ueda, S., Katsura, Y., Orita, K., & Fujimoto, S. (2011). Effects of Acute Prolonged Strenuous Exercise on the Salivary Stress Markers and Inflammatory Cytokines. Jpn J Phys Fitness Sports Med, 60(3), 295–304. https://doi.org/10.7600/jspfsm.60.295
van der Geest, K. S. M., Wang, Q., Eijsvogels, T. M. H., Koenen, H. J. P., Joosten, I., Brouwer, E., … Boots, A. M. H. (2017). Changes in peripheral immune cell numbers and functions in octogenarian walkers - an acute exercise study. Immunity and Ageing, 14(1), 1–13. https://doi.org/10.1186/s12979-017-0087-2
Voorrips, L. E., Ravelli, A. C., Dongelmans, P. C., Deurenberg, P., & van Staveren, W. A. (1991). A physical activity questionnaire for the elderly 108. Med.Sci Sports Exerc., 23(0195–9131 (Print)), 974–979. https://doi.org/10.1249/00005768-199108000-00015
WHO. (2015). World population, ageing. United Nations, Department of Economic and Social Affairs, Population Division (2015). World Population Ageing, United Nat((ST/ESA/SER.A/390), 164. https://doi.org/ST/ESA/SER.A/390
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