Methodological validation of a vertical ladder with low intensity shock stimulus for resistance training in C57BL/6 mice: Effects on muscle mass and strength, body composition, and lactate plasma levels


  • Vinicius Dias Rodrigues State University of Montes Claros (Unimontes), Brazil
  • Daniel de Moraes Pimentel State University of Montes Claros (Unimontes), Brazil
  • Andréia de Souza Brito State University of Montes Claros (Unimontes), Brazil
  • Magda Mendes Vieira State University of Montes Claros (Unimontes), Brazil
  • Amanda Rodrigues Santos State University of Montes Claros (Unimontes), Brazil
  • Amanda Souto Machado State University of Montes Claros (Unimontes), Brazil
  • Lorrane Katherine Martins Pereira State University of Montes Claros (Unimontes), Brazil
  • Fernanda Santos Soares State University of Montes Claros (Unimontes), Brazil
  • Emisael Stênio Batista Gomes State University of Montes Claros (Unimontes), Brazil
  • Mariana Rocha Alves Federal University Fluminense (UFF), Brazil
  • Ludmilla Regina de Souza State University of Montes Claros (Unimontes), Brazil
  • Ricardo Cardoso Cassilhas Federal University of Vales do Jequitinhonha e Mucuri (UFVJM), Brazil
  • Renato Sobral Monteiro Júnior State University of Montes Claros (Unimontes), Brazil
  • Alfredo Maurício Batista De-Paula State University of Montes Claros (Unimontes), Brazil



Resistance exercise, Physical exercise, Rodents, Shock, Climbing


The objective of this study was to evaluate the effects of a vertical ladder device for resistance exercises with or without electrical shock stimulus on muscle strength, body composition, limb volume, muscle fibres and plasma lactate and glycemia of female mice. This device is represented by a vertical ladder with electrostimulation. It was analysed in groups of C57BL/6 mice practicing spontaneous physical activity in enriched environment, practicing resisted climbing exercises, practicing resistance exercises with the utility model in question and controls. The acute effects of blood lactate and dark light-box behaviour, and the short-term chronic effects of muscle strength, limb volume, body composition, muscle fibre area, and central and light-dark quantification were verified. According to the findings, the vertical electrostimulation ladder model presented acute effects on lactate levels, similar to other experimental models of resistance exercise and physical activity. The behaviour in the light-dark box test showed no difference between the groups. Regarding the short-term chronic response, the best results were obtained in the impact-stimulated resistive exercise in the limb traction muscle variables, greater brown adipose tissue weight, greater quadriceps femoral muscle structure, limb and greater weight number of nuclei in the skeletal striated muscle fibres. The use of the prototype showed similarities in the acute and chronic adaptations expected in resistance training. However, new study proposals should be encouraged, as the data presented here are the first notes on the use of this utility model.


Foundation for Research Support of the Minas Gerais State (FAPEMIG), National Council for Scientific and Technological Development (CNPq), Coordination of Improvement of Higher Education Personnel (CAPES)


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How to Cite

Rodrigues, V. D., de Moraes Pimentel, D., de Souza Brito, A., Mendes Vieira, M., Rodrigues Santos, A., Souto Machado, A., Martins Pereira, L. K., Santos Soares, F., Batista Gomes, E. S., Rocha Alves, M., Regina de Souza, L., Cardoso Cassilhas, R., Sobral Monteiro Júnior, R., & Batista De-Paula, A. M. (2019). Methodological validation of a vertical ladder with low intensity shock stimulus for resistance training in C57BL/6 mice: Effects on muscle mass and strength, body composition, and lactate plasma levels. Journal of Human Sport and Exercise, 14(3), 608–631.