Journal of Human Sport and Exercise

Effect of avian immunoglobulin on post-exercise muscle damage and muscular soreness

Bert H. Jacobson, Taylor Monaghan, John Sellers, Carlos Estrada, Masoud Moghaddam



Delayed onset muscular soreness (DOMS) occurs following unaccustomed, strenuous activity and a variety of attempts have been used to reduce or prevent the discomfort and pain of DOMS. This study examined the effect of oral gamma globulin protein (IgY) on post-exercise creatine kinase (CK), C-reactive protein (CRP), and DOMS. In a randomized, double-blind, placebo controlled study, 25 healthy participants were assigned to either IgY or placebo (PL) followed by a blood draw. After 14 days of supplementation, muscle soreness was induced and participants continued to dose and rate DOMS for the next 48 hrs and followed by another blood draw. Analysis revealed that CK levels were significantly (p<0.05) less for the IgY than for the PL group. For CRP there was no significant between group difference, however, the PL group registered a 54.4% increase in C-reactive protein while the IgY group gained 24.4%. The IgY group noted significantly less soreness than the PL at 36 and 48 hrs, suggesting that IgY provides a mechanism that mediates the DOMS inflammatory response. By mediating muscle soreness participants may continue to train and/or compete at a high level without compromising strength, power, or technique.


Muscle; Inflammation; Damage; Soreness; Exercise


Ammar A, Turki M, Chtourou H, Hammouda O, Trabelsi K, Choumous K, Abdelkarim O, Hekelmann A, Bouaziz M, Ayadi F, Driss T, Souissi N. Pomegranate supplementation accelerates recovery of muscle damage and soreness and inflammatory markers after a weightlifting training session. Plos One. October 20, 2016.

Baum K, Telford R, Cunningham R. Marine oil dietary supplementation reduces delayed onset muscle soreness after a 30 km run. J Sports Med 2013; 4:109-115.

Bell P, Stevenson E, Davison G, Howatson G. The effects of Montmorency tart cherry concentrate supplementation on recovery following prolonged, intermittent exercise. Nutrients. 2016; 8: doi:10.3390/nu8070441.

Braun W, Dutto D. The effects of a single bout of downhill running and ensuing delayed onset of muscle soreness on running economy performed 48 h later. Eur J Appl Physiol 2003; 90:29-34.

Brown S, Child Rb, Day Sh, Donnelly Ae. Indices of skeletal muscle damage and connective tissue breakdown following eccentric muscle contractions. Eur J Appl Physiol 1997; 75: 369-374.

Calbet Ja, Chavarren J, Dorado C. Running economy and delayed onset muscle soreness. J Sports Med Phys Fitness 2001; 41: 18-26.

Chazaud B. Inflammation during skeletal muscle regeneration and tissue remodeling: application to exercise –induced muscle damage management. Immunol and Cell Biol. 2016; 94: 140-145.

Chen TC. Variability in muscle damage after eccentric exercise and the repeated bout effect. Res Q Exerc Sport. 2006; 77: 362–371.

Cheung K, Hume P, Maxwell L. Delayed onset muscle soreness: treatment strategies and performance factors. Sports Med. 2003; 33: 145-164.

Close G, Ashton T, Cable T, Doran D, Holloway C, Mcardle F, Maclaren Dpm. Ascorbic acid supplementation does not attenuate post-exercise muscle soreness following muscle-damaging exercise but may delay the recovery process. Br J Nutr. 2006; 95: 976-981.

Connolly DJ, Lauzon C, Agnew J, Dunn M, Reed B. The effects of vitamin C supplementation on symptoms of delayed onset muscle soreness. J Sports Med Phys Fitness. 2006; 46: 462-467.

Damas F, Phillips S, Lixandrão M, Vechin Fc, Libardi Ca, Roschel H, Tricoli V, Ugrinowitsch C. Early resistance training-induced increases in muscle cross-sectional area are concomitant with edema-induced muscle swelling. Eur J Appl Physiol. 2016; 116: 49-56.

Dilorenzo Fm, Drager Cj, Rankin Jw. Docosahexaenoic acid affects markers of inflammation and muscle damage after eccentric exercise. J Strength Cond Res. 2014; 28: 2768-2774.

Fridén J, Sjöström M, Ekblom B. Myofibrillar damage following intense eccentric exercise in man. Int J Sports Med. 1983; 4: 170-176.

Gujral N, Suh Jw, Sunwoo Hh. Effect of anti-gliadin IgY antibody on epithelial intestinal integrity and inflammatory response induced by gliadin. BMC Immunol. 2015; 16:41.

Harris Dj, Atkinson G. Ethical standards in sports and exercise science research: 2016 update. Int J Sports Med. 2015; 26:1121-1124.

Hasenoehrl T, Wessner B, Tschan H, Vidotto C, Crevenna R, Csapo, R. Eccentric resistance training intensity may affect the severity of exercise induced muscle damage. J Sports Med Phys Fitness. 2017: 57: 1195-204.

Hurley C, Hatfield D, Riebe D. The effect of caffeine ingestion on delayed onset muscle soreness. J Strength Cond Res. 2013; 27: 3101-3109.

Hutchison At, Flieller Eb, Dillon Kj, Leverett, Bd. Black currant nectar reduces muscle damage and inflammation following a bout of high-intensity eccentric contractions. J Diet Suppl. 2016; 13:1-15.

Johnsen Sh, Wilsgaard T, Bekkelund Si. Creatine kinase activity and blood pressure in a normal population: the Tromsø study. J Hypertens. 2011; 29: 36–42.

Kato K, Sakamoto T, Ito K. Gamma-globulin inhibits superantigen-induced lymphocyte proliferation and cytokine production. Allergol Int. 2007; 56: 439-444.

Kim Sk, Kim Mc. The effect on delayed onset muscle soreness recovery for ultrasound with bee venom. J Phys Ther Sci. 2014; 26: 1419-1421.

Li X, Yao Y, Wang X, Ahen Y, Thancker Pa, Wang L, Shi M, Shao J, Zong Y, Wang N, Xu Y. Chicken egg yolk antibodies (IgY) modulate the intestinal mucosal immune response in a mouse model of Salmonella typhimurium infection. Int Immunopharmacol. 2016; 36: 305-314.

Linzer P, Filip, M, Šámal F, Kremr J, Šálek T, Gajdos, Jarkovsky J. Comparison of biochemical markers of muscle damage and inflammatory response between the open discectomy, microsurgical discectomy, and microsurgical discectomy using tubular retractor. J Neurol Surg A Cent Eur Neurosurg. 2015; 76(5): 384-391.

Luden Nd, Saunders Mj, Todd Mk. Post-exercise carbohydrate-protein- antioxidant ingestion decreases plasma creatine kinase and muscle soreness. Int J Sport Nutr Exerc Metab. 2007; 17, 109-123.

Lux A, Aschermann S, Biburger M, Nimmerjahn F. The pro and anti-inflammatory activities of immunoglobulin G. Ann Rheum Dis. 2010; 69: i92-i96.

Mackey Al, Bojsen-Moller J, Qvortrup K, Langberg H, Suetta C, Kalliokoski Kk, Kjaer M, Magnusson Sp. Evidence of skeletal muscle damage following electrically stimulated isometric muscle contractions in humans. J Appl Physiol. 2008; 105: 1620-1627.

Manimmanakorn N, Manimmanakorn A, Boobphachart D, Thuwakum W, Laupattarakasem W, Hamlin Mj. Effects of Zingiber cassumunar (Plai cream) in the treatment of delayed onset muscle soreness. J Integr Med. 2016; 14: 114-120.

Mashhadi Ns, Ghiasvand R, Askari G, Hairi M, Askari G, Feizi A, Darvishi L, Hajishaflee M, Barani A. Influence of ginger and cinnamon intake on inflammation and muscle soreness endued by exercise in Iranian female athletes. Int J Prev Med. 2013; 4: S11-S15.

Matsumoto K, Koba T, Hamada K, Sakurai M, Higuchi T, Miyata H. Branched-chain amino acid supplementation attenuates muscle soreness, muscle damage and inflammation during an intensive training program. J Sports Med Phys Fitness. 2009; 49: 424-431.

Matsumura Md, Zavorsky Gs, Smoliga Jm. The effects of pre-exercise ginger supplementation on muscle damage and delayed onset muscle soreness. Phytother Res. 2015; 29: 887-893.

Meamarbashi A, Rajabi A. Preventive effects of 10-day supplementation with saffron and indomethacin on the delayed-onset muscle soreness. Clin J Sport Med. 2015; 25: 105-112.

Müller S, Schubert A, Zajac J, Dyck T, Oelkrug C. IgY antibodies in human nutrition for disease prevention. J Nutr. 2015; 14: 109.

Munhoz, Ls, Vargas, Gdá, Fischer, G, Lima, Md, Esteves, Pa, Hübner, Sdo. Avian IgY antibodies: characteristics and applications in immunodiagnostic. Ciência Rural. 2014; 44(1), 153-160.

Nguyen D, Brown Le, Coburn Jw, Judelson Da, Eurich Ad, Khamoul Av, Uribe Bp. Effect of delayed-onset muscle soreness on elbow flexion strength and rate of velocity development. J Strength Cond Res. 2009; 23: 1282-1286.

Nicol Lm, Rowlands Ds, Fazakerly R, Kellett J. Curcumin supplementation likely attenuates delayed onset muscle soreness (DOMS). Eur J Appl Physiol. 2015; 115: 1769-1777.

Nieman D, Luo B, Dreau D, Henson Da, Shanely Ra, Dew D, Meaney Mp. Immune and inflammation responses to a 3-day period of intensified running versus cycling. Brain Behav Immun. 2014; 39: 180-185.

Nosaka K, Sakamoto K. Effect of elbow joint angle on the magnitude of muscle damage to the elbow flexors. Med Sci Sports Exerc. 2001; 33: 22–29.

Papacosta E, Nassis Gp, Gleeson M. Effects of acute postexercise chocolate milk consumption during intensive judo training on the recovery of salivary hormones, salivary SIgA, mood state, muscle soreness, and judo-related performance. Appl Physiol Nutr Metab. 2015; 40: 1116-1122.

Peake Jm, Neubauer O, Della Gatta Pa, Nosaka K. Muscle damage and inflammation during recovery from exercise. J Appl Physiol. 2017; 122: 559–570.

Pumpa Kl, Fallon Ke, Bensoussan A, Papalia S. The effects of Panax notoginseng on delayed onset muscle soreness and muscle damage in well-trained males: a double blind randomised controlled trial. Complement Ther Med. 2013; 21: 131-140.

Ra S, Akazawa N, Choi Y, Matsubara T, Oikawa S, Kumagai H, Tanahashi K, Ohmori H, Maeda S. Taurine supplementation reduces eccentric exercise-induced delayed onset muscle soreness in young men. Adv Exp Med Biol. 2015; 803: 765-772.

Rahman S, Van Iguyen S, Icatlo Fj, Umeda K, Kodama Y. Oral passive Igy-based immunotherapeutics: a novel solution for prevention and treatment of alimentary tract disease. Hum Vaccin Immunother. 2013; 9(5): 1039-1048.

Rynders Ca, Weltman Jy, Rynders Sd, Patrie J, Mcknight J, Katch Fi, Hertel J, Weltman A. Effect of an herbal/botanical supplement on recovery from delayed onset muscle soreness: a randomized placebo-controlled trial. J Int Soc Sports Nutr. 2014; 1127.

Souglis A, Bogdanis Gc, Giannopoulou I, Papadopoulos C, Apostolidis N. Comparison of inflammatory responses and muscle damage indices following a soccer, basketball, volleyball and handball game at an elite competitive level. Res Sports Med. 2015; 23: 59-72.

Svage Kj, Clarkson Pm. Oral contraceptive use and exercise-induced muscle damage and recovery. Contraception. 2002; 66: 67-71.

Tidball Jg, St Pierre Ba. Apoptosis of macrophages during the resolution of muscle inflammation. J Leukoc Biol. 1996; 59: 380-388.

Tofas T, Jamurtas Az, Fatouros I, Nikolaidis Mg, Koutedeakis Y, Papageorgakopolus N, Theocharis Da. Plyometric exercise increases serum indices of muscle damage and collagen breakdown. J Strength Cond Res. 2008; 22: 490-496.

Tojima M, Noma K, Torii S. Changes in serum creatine kinase, leg muscle tightness, and delayed onset muscle soreness after a full marathon race. J Sports Med Phys Fitness. 2016; 56: 782-788.

Wei-Xu H, Wen-Yun Z, Xi-Ling Z, Shu W, Li-Hua W, Xiao-Mu W, Hui-Ping W, Wen-Ding W, Dan H, Qin Z, Gou-Zhu H. Anti-Interleukin-1 beta/tumor necrosis factor-alpha IgY antibodies reduce pathological allergic responses in guinea pigs with allergic rhinitis. Mediators Inflamm. 2016; 3128182: 1-11.


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