The beneficial effects of physical activity and weight loss on human colorectal carcinoma cell lines
DOI:
https://doi.org/10.14198/jhse.2020.15.Proc2.16Keywords:
Physical activity, Obesity, Colorectal cancer, IL-6, IL-8, IL-10Abstract
Recent studies have demonstrated that obesity is a significant risk factor for the development of several malignancies such as cancer. Colorectal cancer is among the most common cancers worldwide and is strong linked to obesity. A healthy lifestyle, characterized by hypocaloric diet and physical activity, is important to reduce a chronic inflammation, oxidative stress and metabolic disorders typical of obesity (Messina et al, 2018; Messina et al, 2017; Messina et al, 2015). It is well known that the chronic inflammation state and oxidative stress are responsible for the aging and development of many diseases, such as cancer. Dysregulation of cytokine’s secretion probably participates in the establishment of cancer in obese patients. The aim of this study is to analyse the effects of sera from obese patients subjected to a physical activity program before and after weight loss on cell viability, apoptosis and oxidative stress in HCT116 carcinoma cell line treated for 24, 48 and 72 hours through MTT test. We analysed the expression of cytokines in HCT116 cells. We found that sera from obese after physical activity intervention compared to treatment with sera from obese patients before physical activity intervention reduce the survival rate of HCT116 cells through induction of apoptosis and oxidative stress. Finally, we found a reduction of mRNA levels corresponding to the pro-inflammatory IL-6 and IL-8 cytokines together with an increase of the anti-inflammatory IL-10 cytokine. We can conclude that the physical activity has numerous beneficial effects also in colorectal cancer cell, indeed the physical activity and weight loss in obese subjects have an inhibitory and anti-inflammatory effects in a short period on carcinoma cell line.
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Bianco A, Gentile A, Boca S, et al. (2018). An exploratory analysis of factors associated with health-related physical fitness in adolescents. Sustainability; 10:1847. https://doi.org/10.3390/su10061847
Boutari C, Perakakis N, Mantzoros CS. (2018). Association of Adipokines with Development and Progression of Nonalcoholic Fatty Liver Disease. Endocrinol Metab. 33(1):33-43. https://doi.org/10.3803/enm.2018.33.1.33
Bray G.A, Kim K.K, Wilding JPH. (2017). Obesity: a chronic relapsing progressive disease process. A position statement of the World Obesity Federation. Obes Rev. 18(7):715-723. https://doi.org/10.1111/obr.12551
Carpagnano GE, Sessa F, Scioscia G, Lacedonia D, Foschino MP, Venuti MP, Triggiani AI, Valenzano A, Resta O, Cibelli G, Messina G. (2020). Physical Activity as a New Tool to Evaluate the Response to Omalizumab and Mepolizumab in Severe Asthmatic Patients: A Pilot Study. Front Pharmacol.10:1630. https://doi.org/10.3389/fphar.2019.01630
Chieffi S, Carotenuto M, Monda V, Valenzano A, Villano I, Precenzano F, et al. (2017). Orexin System: The Key for a Healthy Life. Front. Physiol. 8:357. https://doi.org/10.3389/fphys.2017.00357
Corbi G, Polito R, Monaco ML, Cacciatore F, Scioli M, Ferrara N, Daniele A, Nigro E. (2019). Adiponectin Expression and Genotypes in Italian People with Severe Obesity Undergone a Hypocaloric Diet and Physical Exercise Program. Nutrients. 11(9). https://doi.org/10.3390/nu11092195
Cristian V Francavilla, Francesco Sessa, Monica Salerno, Giuseppe D Albano, Ines Villano, et al. (2018). Influence of Football on Physiological Cardiac Indexes in Professional and Young Athletes. Front Physiol. 9:153. https://doi.org/10.3389/fphys.2018.00153
Di Mauro R, Cantarella G, Bernardini R, Di Rosa M, Barbagallo I, Distefano A, Longhitano L, Vicario N, Nicolosi D, Lazzarino G, Tibullo D, Gulino ME, Spampinato M, Avola R, Li Volti G. (2019). The Biochemical and Pharmacological Properties of Ozone: The Smell of Protection in Acute and Chronic Diseases. Int J Mol Sci. 20(3). pii: E634. https://doi.org/10.3390/ijms20030634
Di Meo S, Napolitano G, Venditti P. (2019). Mediators of Physical Activity Protection against ROS-Linked Skeletal Muscle Damage. Int J Mol Sci. 20(12). pii: E3024. https://doi.org/10.3390/ijms20123024
Di Zazzo E, Polito R, Bartollino S, Nigro E, Porcile C, Bianco A, Daniele A, Moncharmont B. (2019). Adiponectin as Link Factor between Adipose Tissue and Cancer. Int J Mol Sci. 20(4). pii: E839. https://doi.org/10.3390/ijms20040839
Dore MP, Longo NP, Manca A, Pes GM. (2020). The impact of body weight on dysplasia of colonic adenomas: a case-control study. Scand J Gastroenterol. 2020 Apr 1:1-6. https://doi.org/10.1080/00365521.2020.1746393
Francavilla G, Francavilla C. Physical exercise is therapy. MED SPORT 2013;66:625-8.
Francavilla VC, Bongiovanni T, Genovesi F, Minafra P, Francavilla G. (2015). Localized bioelectrical impedance analysis: how useful is it in the follow-up of muscle injury? A case report. MED SPORT; 68:323-34.
Francavilla VC, Bongiovanni T, Todaro L, Di Pietro V, Francavilla G. (2017). Probiotic supplements and athletic performance: a review of the literature. MED SPORT; 70:000-000. https://doi.org/10.23736/S0025-7826.17.03037-X
Greco T, Glenn T, Hoyda D, Prins LM. (2016). Ketogenic diet decreases oxidative stress and improves mitochondrial respiratory complex activity. J Cereb Blood Flow Metab. https://doi.org/10.1177/0271678x15610584
Horvath S, Raj K. (2018). DNA methylation-based biomarkers and the epigenetic clock theory of ageing. Nat Rev Genet. 19(6):371-384. https://doi.org/10.1038/s41576-018-0004-3
Jornayvaz FR, Jurczak MJ, Lee HY, Birkenfeld AL, Frederick DW, Zhang D, Zhang XM, Samuel VT, Shulman GI. (2010). A high-fat, ketogenic diet causes hepatic insulin resistance in mice, despite increasing energy expenditure and preventing weight gain. Am. J. Physiol. Endocrinol. Metab. 299:E808–E815. https://doi.org/10.1152/ajpendo.00361.2010
Larsen S, Dandanell S, Kristensen KB, Jørgensen SD, Dela F, Helge JW. (2019). Influence of exercise amount and intensity on long-term weight loss maintenance and skeletal muscle mitochondrial ROS production in humans. Appl Physiol Nutr Metab. 44(9):958-964. https://doi.org/10.1139/apnm-2018-0577
Mahmoud Al-Dayyat , H., Rayyan, Y.M., Tayyem, R.F. (2018). Relationship of serum leptin with some biochemical, anthropometric parameters and abdominal fat volumes as measured by magnetic resonance imaging. Diabetes Metab Syndr, 22;12(3):207-213. https://doi.org/10.1016/j.dsx.2017.09.004
Mauri E, Sacchetti A, Vicario N, Peruzzotti-Jametti L, Rossi F, Pluchino S. (2018). Evaluation of RGD functionalization in hybrid hydrogels as 3D neural stem cell culture systems. Biomater Sci, 6(3):501-510. https://doi.org/10.1039/c7bm01056g
Mazzeo F, Motti ML, Messina G, et al. (2013). Use of nutritional supplements among south Italian students of Physical Training and Sport University. Current Topics in Toxicology. 9:21-26.
Messina A, Bitetti I, Precenzano F, Iacono D, Messina G, Roccella M, Parisi. et al. (2018). Non-Rapid Eye Movement Sleep Parasomnias and Migraine: A Role of Orexinergic Projections. Front Neurol.;9:95. https://doi.org/10.3389/fneur.2018.00095
Messina A, Monda M, Valenzano A, Messina G, Villano I, Moscatelli F, Cibelli G, Marsala G, Polito R, Ruberto M, Carotenuto M, Monda V, Viggiano A, Daniele A, Nigro E. (2018). Functional Changes Induced by Orexin A and Adiponectin on the Sympathetic/Parasympathetic Balance. Front Physiol. 9:259. https://doi.org/10.3389/fphys.2018.00259
Messina A, Monda V, et al. (2017). Role of the orexin system on arousal, attention, feeding behaviour and sleep disorders. Acta Medica Mediterr. 33(4):645-64 https://doi.org/10.19193/0393-6384_2017_4_096
Messina G, Zannella C, Monda V, Dato A, Liccardo D, De Blasio S, Valenzano A, Moscatelli F, Messina A, Cibelli G and Monda M. (2015). The Beneficial Effects of Coffee in Human Nutrition. Biol Med. https://doi.org/10.4172/0974-8369.1000240
Monda V, La Marra M, Perrella R, Caviglia G, Iavarone A, Chieffi S, Messina G, Carotenuto M, Monda M, Messina A. (2017). Obesity and brain illness: from cognitive and psychological evidences to obesity paradox. Diabetes Metab Syndr Obes.;10:473-479. https://doi.org/10.2147/dmso.s148392
Moreno B, Crujeiras AB, Bellido D, Sajoux I, Casanueva FF. (2016). Obesity treatment by very low-calorie-ketogenic diet at two years: reduction in visceral fat and on the burden of disease. Endocrine. 3:681-690. https://doi.org/10.1007/s12020-016-1050-2
Moro T, Tinsley G, Bianco A, Marcolin G, Pacelli QF, Battaglia G, et al. (2016). Effects of eight weeks of time-restricted feeding (16/8) on basal metabolism, maximal strength, body composition, inflammation, and cardiovascular risk factors in resistance-trained males. J Transl Med. 13;14(1):290. https://doi.org/10.1186/s12967-016-1044-0
Moscatelli F, Messina G, Valenzano A, Monda M, et al. (2015). Relationship between RPE and Blood Lactate after Fatiguing Handgrip Exercise in Taekwondo and Sedentary Subjects. Biol Med. https://doi.org/10.4172/0974-8369.1000s3008
Moscatelli F, Messina G, Valenzano A, Monda V, Viggiano A, Messina A, Petito A, Triggiani AI, Ciliberti MA, Monda M, Capranica L, Cibelli G. (2016). Differences in corticospinal system activity and reaction response between karate athletes and non-athletes. Neurological Sciences. 37(12). https://doi.org/10.1007/s10072-016-2693-8
Newsholme P, Cruzat VF, Keane KN, Carlessi R, de Bittencourt PI Jr. (2016). Molecular mechanisms of ROS production and oxidative stress in diabetes. Biochem J. 473(24):4527-4550. https://doi.org/10.1042/bcj20160503c
Nigro E, Sangiorgio D, Scudiero O, Monaco ML, Polito R, Villone G, Daniele A. (2016). Gene molecular analysis and Adiponectin expression in professional Water Polo players. Cytokine.; 81:88-93. https://doi.org/10.1016/j.cyto.2016.03.002
Nigro E, Schettino P, Polito R, Scudiero O, Monaco ML, De Palma GD, Daniele A. (2018). Adiponectin and colon cancer: evidence for inhibitory effects on viability and migration of human colorectal cell lines. Mol Cell Biochem. 448(1-2):125-135. https://doi.org/10.1007/s11010-018-3319-7
Panico A, Messina G, Lupoli GA, Lupoli R, Cacciapuoti M, Moscatelli F, Esposito T, Villano I, Valenzano A, Monda V, Messina A, Precenzano F, Cibelli G, Monda M, Lupoli G. (2017). Quality of life in overweight (obese) and normal-weight women with polycystic ovary syndrome. Patient Prefer Adherence. 11:423-429. https://doi.org/10.2147/ppa.s119180
Patti A, Bianco A, Karsten B, Montalto MA, Battaglia G, et al. (2017). The effects of physical training without equipment on pain perception and balance in the elderly: A randomized controlled trial. Work; 57:23-30. https://doi.org/10.3233/wor-172539
Polito R, Nigro E, Messina A, Monaco ML, Monda V, Scudiero O, Cibelli G, Valenzano A, Picciocchi E, Zammit C, Pisanelli D, Monda M, Cincione IR, Daniele A, Messina G. (2018). Adiponectin and Orexin-A as a Potential Immunity Link Between Adipose Tissue and Central Nervous System. Front Physiol.; 9:982. https://doi.org/10.3389/fphys.2018.00982
Sessa F, Anna V, Messina G, Cibelli G, Monda V, Marsala G, Ruberto M, Biondi A, Cascio O, Bertozzi G, Pisanelli D, Maglietta F, Messina A, Mollica MP, Salerno M. (2018). Heart rate variability as predictive factor for sudden cardiac death. Aging; 23;10(2):166-177. https://doi.org/10.18632/aging.101386
Valenzano A, Polito R, Trimigno V, Di Palma A, Moscatelli F, Corso G, Sessa F, Salerno M, Montana A, Di Nunno N, Astuto M, Daniele A, Carotenuto M, Messina G, Cibelli G, Monda V. (2019). Effects of Very Low Calorie Ketogenic Diet on the Orexinergic System, Visceral Adipose Tissue, and ROS Production. Antioxidants. 8(12). pii: E643. https://doi.org/10.3390/antiox8120643
Vicario N, Pasquinucci L, Spitale FM, Chiechio S, Turnaturi R, Caraci F, Tibullo D, Avola R, Gulino R, Parenti R, Parenti C. (2019). Simultaneous Activation of Mu and Delta Opioid Receptors Reduces Allodynia and Astrocytic Connexin 43 in an Animal Model of Neuropathic Pain. Mol Neurobiol. 56(11):7338-7354. https://doi.org/10.1007/s12035-019-1607-1
Viggiano A, Vicidomini C, Monda M, Carleo D, Carleo R, Messina G, Viggiano A, Viggiano E, De Luca B. (2009). Fast and low-cost analysis of heart rate variability reveals vegetative alterations in noncomplicated diabetic patients. J Diabetes Complications. 23(2):119-23. https://doi.org/10.1016/j.jdiacomp.2007.11.009
Viggiano E, Monda V, Messina A, Moscatelli F, Valenzano A, Tafuri D, Cibelli G, De Luca B, Messina G, Monda M. (2016). Cortical spreading depression produces a neuroprotective effect activating mitochondrial uncoupling protein-5. Neuropsychiatr Dis Treat. 11;12:1705-10. https://doi.org/10.2147/ndt.s107074
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