The beneficial effects of physical activity and weight loss on human colorectal carcinoma cell lines


  • Rita Polito University of Foggia, Italy
  • Alessia Scarinci University of Bari, Italy
  • Antonio Ambrosi University of Foggia, Italy
  • Nicola Tartaglia University of Foggia, Italy
  • Domenico Tafuri University of Naples "Parthenope", Italy
  • Marcellino Monda University of Campania Luigi Vanvitelli, Italy
  • Antonietta Messina University of Campania Luigi Vanvitelli, Italy
  • Fabiano Cimmino University of Naples Federico II, Italy
  • Angela Catapano University of Naples Federico II, Italy
  • Francesco Sessa University of Foggia, Italy
  • Girolamo Di Maio University of Campania Luigi Vanvitelli, Italy
  • Vincenzo Cristian Francavilla Kore University of Enna, Italy
  • Giovanni Messina University of Foggia, Italy
  • Vincenzo Monda University of Campania Luigi Vanvitelli, Italy



Physical activity, Obesity, Colorectal cancer, IL-6, IL-8, IL-10


Recent studies have demonstrated that obesity is a significant risk factor for the development of several malignancies such as cancer. Colorectal cancer is among the most common cancers worldwide and is strong linked to obesity. A healthy lifestyle, characterized by hypocaloric diet and physical activity, is important to reduce a chronic inflammation, oxidative stress and metabolic disorders typical of obesity (Messina et al, 2018; Messina et al, 2017; Messina et al, 2015). It is well known that the chronic inflammation state and oxidative stress are responsible for the aging and development of many diseases, such as cancer. Dysregulation of cytokine’s secretion probably participates in the establishment of cancer in obese patients. The aim of this study is to analyse the effects of sera from obese patients subjected to a physical activity program before and after weight loss on cell viability, apoptosis and oxidative stress in HCT116 carcinoma cell line treated for 24, 48 and 72 hours through MTT test. We analysed the expression of cytokines in HCT116 cells. We found that sera from obese after physical activity intervention compared to treatment with sera from obese patients before physical activity intervention reduce the survival rate of HCT116 cells through induction of apoptosis and oxidative stress. Finally, we found a reduction of mRNA levels corresponding to the pro-inflammatory IL-6 and IL-8 cytokines together with an increase of the anti-inflammatory IL-10 cytokine. We can conclude that the physical activity has numerous beneficial effects also in colorectal cancer cell, indeed the physical activity and weight loss in obese subjects have an inhibitory and anti-inflammatory effects in a short period on carcinoma cell line.


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How to Cite

Polito, R., Scarinci, A., Ambrosi, A., Tartaglia, N., Tafuri, D., Monda, M., Messina, A., Cimmino, F., Catapano, A., Sessa, F., Di Maio, G., Francavilla, V. C., Messina, G., & Monda, V. (2020). The beneficial effects of physical activity and weight loss on human colorectal carcinoma cell lines. Journal of Human Sport and Exercise, 15(2proc), S252-S260.

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