Global DNA methylation and physical fitness of elderly athletes with lifelong endurance activity
DOI:
https://doi.org/10.14198/jhse.2021.164.15Keywords:
Global DNA methylation, Physical fitness, Ageing, EnduranceAbstract
Background: Level of Global DNA methylation is associated with many diseases and the influence of physical activity is being investigated by several research groups. The aim of our study was to assess the effect of lifetime endurance physical activity on global DNA methylation, physical fitness and body composition. Methods: A total of 31 elderly males were involved in the study, divided into two groups based on differences in physical activity. The first group consisted of 18 volunteers with lifetime endurance activity (mean age: 65.1 ± 3.3 yr.; height: 174.8 ± 4.9; weight: 81.5 ± 6,1 kg; BMI: 24.2 ± 1.1). The control group consisted of thirteen elderly individuals with a sedentary lifestyle (mean age: 64.8 ± 3.1 yr.; height: 176.5 ± 5.5; weight: 87.9 ± 10.1 kg; BMI: 27.8 ± 2.9). Quantification of global DNA methylation was performed in DNA isolated from peripheral blood mononucleated cells by LINE-1 pyrosequencing. Results: Elderly individuals with lifetime endurance activity had a better level of physical fitness VO2max on average 30 % (35.7 ± 10.6 vs. 31.9 ± 7,7 ml.kg-1.min-1, p <.01) and lower mean body fat content (17.46 ± 2.52 vs. 27.8 ± 2.9 %, p < .01). Global DNA methylation did not differ between studied groups (81.1 ± 2.1 vs. 80.5 ± 1.6 %). Conclusion: Better level of physical fitness does not influence the level of the global DNA methylation in peripheral blood mononuclear cells significantly. For future research we recommended to observe DNA methylation changes in specific tissues (e.g. skeletal muscle fibres).Funding
Slovak Research and Development Agency, Ministry of Education, Science, Research and Sport of the Slovak RepublicDownloads
References
Anderson, O. S., Sant, K. E., & Dolinoy, D. C. (2012). Nutrition and epigenetics: an interplay of dietary methyl donors, one-carbon metabolism and DNA methylation. Journal of Nutritional Biochemistry, 23(8), 853-859. https://doi.org/10.1016/j.jnutbio.2012.03.003
Arnett, S. W., Laity, J. H., Agrawal, S. K., & Cress, M. E. (2008). Aerobic reserve and physical functional performance in older adults. Age Ageing, 37(4), 384-389. https://doi.org/10.1093/ageing/afn022
Avery, O. T., Macleod, C. M., & McCarty, M. (1944). Studies on the Chemical Nature of the Substance Inducing Transformation of Pneumococcal Types : Induction of Transformation by a Desoxyribonucleic Acid Fraction Isolated from Pneumococcus Type Iii. J Exp Med, 79(2), 137-158. https://doi.org/10.1084/jem.79.2.137
Ballard-Barbash, R., Friedenreich, C. M., Courneya, K. S., Siddiqi, S. M., McTiernan, A., & Alfano, C. M. (2012). Physical Activity, Biomarkers, and Disease Outcomes in Cancer Survivors: A Systematic Review. Jnci-Journal of the National Cancer Institute, 104(11), 815-840. https://doi.org/10.1093/jnci/djs207
Bannister, A. J., & Kouzarides, T. (2011). Regulation of chromatin by histone modifications. Cell Research, 21(3), 381-395. https://doi.org/10.1038/cr.2011.22
Bjornsson, H. T., Sigurdsson, M. I., Fallin, M. D., Irizarry, R. A., Aspelund, T., Cui, H. M., . . . Feinberg, A. P. (2008). Intra-individual change over time in DNA methylation with familial clustering. Jama-Journal of the American Medical Association, 299(24), 2877-2883. https://doi.org/10.1001/jama.299.24.2877
Bollati, V., Schwartz, J., Wright, R., Litonjua, A., Tarantini, L., Suh, H., . . . Baccarelli, A. (2009). Decline in genomic DNA methylation through aging in a cohort of elderly subjects. Mechanisms of Ageing and Development, 130(4), 234-239. https://doi.org/10.1016/j.mad.2008.12.003
Bormann, F., Rodriguez-Paredes, M., Hagemann, S., Manchanda, H., Kristof, B., Gutekunst, J., . . . Lyko, F. (2016). Reduced DNA methylation patterning and transcriptional connectivity define human skin aging. Aging Cell, 15(3), 563-571. https://doi.org/10.1111/acel.12470
Bornhorst, C., Siani, A., Russo, P., Kourides, Y., Sion, I., Molnar, D., Tilling, K. (2016). Early Life Factors and Inter-Country Heterogeneity in BMI Growth Trajectories of European Children: The IDEFICS Study. Plos One, 11(2). https://doi.org/10.1371/journal.pone.0149268
Boyne, D. J., O'Sullivan, D. E., Olij, B. F., King, W. D., Friedenreich, C. M., & Brenner, D. R. (2018). Physical Activity, Global DNA Methylation, and Breast Cancer Risk: A Systematic Literature Review and Meta-analysis. Cancer Epidemiol Biomarkers Prev, 27(11), 1320-1331. https://doi.org/10.1158/1055-9965.EPI-18-0175
Brennan, K., & Flanagan, J. M. (2012). Is There a Link Between Genome-Wide Hypomethylation in Blood and Cancer Risk? Cancer Prevention Research, 5(12), 1345-1357. https://doi.org/10.1158/1940-6207.CAPR-12-0316
Brooks, W. H., Le Dantec, C., Pers, J. O., Youinou, P., & Renaudineau, Y. (2010). Epigenetics and autoimmunity. Journal of Autoimmunity, 34(3), J207-J219. https://doi.org/10.1016/j.jaut.2009.12.006
Cheung, H. H., Lee, T. L., Davis, A. J., Taft, D. H., Rennert, O. M., & Chan, W. Y. (2010). Genome-wide DNA methylation profiling reveals novel epigenetically regulated genes and non-coding RNAs in human testicular cancer. British Journal of Cancer, 102(2), 419-427. https://doi.org/10.1038/sj.bjc.6605505
Chouliaras, L., Rutten, B. P. F., Kenis, G., Peerbooms, O., Visser, P. J., Verhey, F., . . . van den Hove, D. L. A. (2010). Epigenetic regulation in the pathophysiology of Alzheimer's disease. Progress in Neurobiology, 90(4), 498-510. https://doi.org/10.1016/j.pneurobio.2010.01.002
Dawson, M. A., & Kouzarides, T. (2012). Cancer epigenetics: from mechanism to therapy. Cell, 150(1), 12-27. https://doi.org/10.1016/j.cell.2012.06.013
Ehrlich, M. (2002). DNA methylation in cancer: too much, but also too little. Oncogene, 21(35), 5400-5413. https://doi.org/10.1038/sj.onc.1205651
Ferioli, M., Zauli, G., Maiorano, P., Milani, D., Mirandola, P., & Neri, L. M. (2019). Role of physical exercise in the regulation of epigenetic mechanisms in inflammation, cancer, neurodegenerative diseases, and aging process. Journal of Cellular Physiology, 234(9), 14852-14864. https://doi.org/10.1002/jcp.28304
Gomes, M. V. M., Toffoli, L. V., Arruda, D. W., Soldera, L. M., Pelosi, G. G., Neves-Souza, R. D., . . . Marquez, A. S. (2012). Age-Related Changes in the Global DNA Methylation Profile of Leukocytes Are Linked to Nutrition but Are Not Associated with the MTHFR C677T Genotype or to Functional Capacities. Plos One, 7(12). https://doi.org/10.1371/journal.pone.0052570
Haskell, W. L., Lee, I. M., Pate, R. R., Powell, K. E., Blair, S. N., Franklin, B. A., . . . Bauman, A. (2007). Physical activity and public health - Updated recommendation for adults from the American college of sports medicine and the American heart association. Circulation, 116(9), 1081-1093. https://doi.org/10.1161/CIRCULATIONAHA.107.185649
Ho, S. M. (2010). Environmental epigenetics of asthma: An update. Journal of Allergy and Clinical Immunology, 126(3), 453-465. https://doi.org/10.1016/j.jaci.2010.07.030
Hurley, B. F., & Roth, S. M. (2000). Strength training in the elderly - Effects on risk factors for age-related diseases. Sports Medicine, 30(4), 249-268. https://doi.org/10.2165/00007256-200030040-00002
Joyner, M. J., & Green, D. J. (2009). Exercise protects the cardiovascular system: effects beyond traditional risk factors. Journal of Physiology-London, 587(23), 5551-5558. https://doi.org/10.1113/jphysiol.2009.179432
Kanai, Y. (2010). Genome-wide DNA methylation profiles in precancerous conditions and cancers. Cancer Science, 101(1), 36-45. https://doi.org/10.1111/j.1349-7006.2009.01383.x
Kawakami, R., Kashino, I., Kasai, H., Kawai, K., Li, Y. S., Nanri, A., . . . Mizoue, T. (2019). Leisure-time physical activity and DNA damage among Japanese workers. Plos One, 14(2). https://doi.org/10.1371/journal.pone.0212499
Kell, R. T., & Asmundson, G. J. G. (2009). A Comparison of Two Forms of Periodized Exercise Rehabilitation Programs in the Management of Chronic Nonspecific Low-Back Pain. Journal of Strength and Conditioning Research, 23(2), 513-523. https://doi.org/10.1519/JSC.0b013e3181918a6e
Lagerros, Y. T., Mucci, L. A., Bellocco, R., Nyren, O., Balter, O., & Balter, K. A. (2006). Validity and reliability of self-reported total energy expenditure using a novel instrument. European Journal of Epidemiology, 21(3), 227-236. https://doi.org/10.1007/s10654-006-0013-y
La Vecchia, C., Gallus, S., & Garattini, S. (2012). Effects of physical inactivity on non-communicable diseases. Lancet, 380(9853), 1553-1553. https://doi.org/10.1016/S0140-6736(12)61872-8
Lee, I. M., Shiroma, E. J., Lobelo, F., Puska, P., Blair, S. N., Katzmarzyk, P. T., & Workin, L. P. A. S. (2012). Effect of physical inactivity on major non-communicable diseases worldwide: an analysis of burden of disease and life expectancy. Lancet, 380(9838), 219-229. https://doi.org/10.1016/S0140-6736(12)61031-9
Luttropp, K., Nordfors, L., Ekstrom, T. J., & Lind, L. (2013). Physical activity is associated with decreased global DNA methylation in Swedish older individuals. Scandinavian Journal of Clinical & Laboratory Investigation, 73(2), 184-185. https://doi.org/10.3109/00365513.2012.743166
Matzke, M. A., & Birchler, J. A. (2005). RNAi-mediated pathways in the nucleus. Nature Reviews Genetics, 6(1), 24-35. https://doi.org/10.1038/nrg1500
Morabia, A., Zhang, F. F., Kappil, M. A., Flory, J., Mirer, F. E., Santella, R. M., . . . Markowitz, S. B. (2012). Biologic and epigenetic impact of commuting to work by car or using public transportation: A case-control study. Preventive Medicine, 54(3-4), 229-233. https://doi.org/10.1016/j.ypmed.2012.01.019
Park, J. H., Cho, H., Shin, J. H., Kim, T., Park, S. B., Choi, B. Y., & Kim, M. J. (2014). Relationship Among Fear of Falling, Physical Performance, and Physical Characteristics of the Rural Elderly. American Journal of Physical Medicine & Rehabilitation, 93(5), 379-386. https://doi.org/10.1097/PHM.0000000000000009
Polli, A., Ickmans, K., Godderis, L., & Nijs, J. (2019). When Environment Meets Genetics: A Clinical Review of the Epigenetics of Pain, Psychological Factors, and Physical Activity. Archives of Physical Medicine and Rehabilitation, 100(6), 1153-1161. https://doi.org/10.1016/j.apmr.2018.09.118
Rogers, M. A., Hagberg, J. M., Martin, W. H., Ehsani, A. A., & Holloszy, J. O. (1990). Decline in VO2max with Aging in Master Athletes and Sedentary Men. Journal of Applied Physiology, 68(5), 2195-2199. https://doi.org/10.1152/jappl.1990.68.5.2195
Romermann, D., Hasemeier, B., Metzig, K., Gohring, G., Schlegelberger, B., Langer, F., Lehmann, U. (2008). Global increase in DNA methylation in patients with myelodysplastic syndrome. Leukemia, 22(10), 1954-1956. https://doi.org/10.1038/leu.2008.76
Sharma, R. P., Gavin, D. P., & Grayson, D. R. (2010). CpG methylation in neurons: message, memory, or mask? Neuropsychopharmacology, 35(10), 2009-2020. https://doi.org/10.1038/npp.2010.85
Spence, R. R., Heesch, K. C., & Brown, W. J. (2011). Colorectal cancer survivors' exercise experiences and preferences: qualitative findings from an exercise rehabilitation programme immediately after chemotherapy. European Journal of Cancer Care, 20(2), 257-266. https://doi.org/10.1111/j.1365-2354.2010.01214.x
Terry, M. B., Delgado-Cruzata, L., Vin-Raviv, N., Wu, H. C., & Santella, R. M. (2011). DNA methylation in white blood cells Association with risk factors in epidemiologic studies. Epigenetics, 6(7), 828-837. https://doi.org/10.4161/epi.6.7.16500
Tra, J., Kondo, T., Lu, Q. J., Kuick, R., Hanash, S., & Richardson, B. (2002). Infrequent occurrence of age-dependent changes in CpG island methylation as detected by restriction landmark genome scanning. Mechanisms of Ageing and Development, 123(11), 1487-1503. https://doi.org/10.1016/S0047-6374(02)00080-5
Tronick, E., & Hunter, R. G. (2016). Waddington, Dynamic Systems, and Epigenetics. Frontiers in Behavioral Neuroscience, 10. https://doi.org/10.3389/fnbeh.2016.00107
Van Roekel, E. H., Dugue, P. A., Jung, C. H., Joo, J. E., Makalic, E., Wong, E. M., . . . Milne, R. L. (2019). Physical Activity, Television Viewing Time, and DNA Methylation in Peripheral Blood. Medicine and Science in Sports and Exercise, 51(3), 490-498. https://doi.org/10.1249/MSS.0000000000001827
Vita, A. J., Terry, R. B., Hubert, H. B., & Fries, J. F. (1998). Aging, health risks, and cumulative disability. New England Journal of Medicine, 338(15), 1035-1041. https://doi.org/10.1056/NEJM199804093381506
Voisin, S., Eynon, N., Yan, X., & Bishop, D. J. (2015). Exercise training and DNA methylation in humans. Acta Physiol (Oxf), 213(1), 39-59. https://doi.org/10.1111/apha.12414
Volkmar, M., Dedeurwaerder, S., Cunha, D. A., Ndlovu, M. N., Defrance, M., Deplus, R.,Fuks, F. (2012). DNA methylation profiling identifies epigenetic dysregulation in pancreatic islets from type 2 diabetic patients. Embo Journal, 31(6), 1405-1426. https://doi.org/10.1038/emboj.2011.503
Walsh, D. W., Green, B. C., Holahan, C., Cance, J. D., & Lee, D. (2019). Healthy aging? An evaluation of sport participation as a resource. https://doi.org/10.1080/00222216.2018.1554092
Waxman, A., & Norum, K. R. (2004). Why a global strategy on diet, physical activity and health? The growing burden of non-communicable diseases. Public Health Nutrition, 7(3), 381-383. https://doi.org/10.1079/PHN2004623
Weber, M., Davies, J. J., Wittig, D., Oakeley, E. J., Haase, M., Lam, W. L., & Schubeler, D. (2005). Chromosome-wide and promoter-specific analyses identify sites of differential DNA methylation in normal and transformed human cells. Nature Genetics, 37(8), 853-862. https://doi.org/10.1038/ng1598
Wen, C. P., & Wu, X. F. (2012). Stressing harms of physical inactivity to promote exercise. Lancet, 380(9838), 192-193. https://doi.org/10.1016/S0140-6736(12)60954-4
Weng, X. L., Liu, F. T., Zhang, H., Kan, M. Y., Wang, T., Dong, M. Y., & Liu, Y. (2018). Genome-wide DNA methylation profiling in infants born to gestational diabetes mellitus. Diabetes Research and Clinical Practice, 142, 10-18. https://doi.org/10.1016/j.diabres.2018.03.016
White, A. J., Sandler, D. P., Bolick, S. C. E., Xu, Z. L., Taylor, J. A., & DeRoo, L. A. (2013). Recreational and household physical activity at different time points and DNA global methylation. European Journal of Cancer, 49(9), 2199-2206. https://doi.org/10.1016/j.ejca.2013.02.013
Woo, H. D., & Kim, J. (2012). Global DNA Hypomethylation in Peripheral Blood Leukocytes as a Biomarker for Cancer Risk: A Meta-Analysis. Plos One, 7(4). https://doi.org/10.1371/journal.pone.0034615
Yu, W. Q., Gius, D., Onyango, P., Muldoon-Jacobs, K., Karp, J., Feinberg, A. P., & Cui, H. M. (2008). Epigenetic silencing of tumour suppressor gene p15 by its antisense RNA. Nature, 451(7175), 202-U210. https://doi.org/10.1038/nature06468
Yuasa, Y., Nagasaki, H., Akiyama, Y., Hashimoto, Y., Takizawa, T., Kojima, K., Nakachi, K. (2009). DNA methylation status is inversely correlated with green tea intake and physical activity in gastric cancer patients. International Journal of Cancer, 124(11), 2677-2682. https://doi.org/10.1002/ijc.24231
Zhang, F. F., Cardarelli, R., Carroll, J., Fulda, K. G., Kaur, M., Gonzalez, K., . . . Morabia, A. (2011). Significant differences in global genomic DNA methylation by gender and race/ethnicity in peripheral blood. Epigenetics, 6(5), 623-629. https://doi.org/10.4161/epi.6.5.15335
Downloads
Statistics
Published
How to Cite
Issue
Section
License
Copyright (c) 2018 Journal of Human Sport and Exercise
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Each author warrants that his or her submission to the Work is original and that he or she has full power to enter into this agreement. Neither this Work nor a similar work has been published elsewhere in any language nor shall be submitted for publication elsewhere while under consideration by JHSE. Each author also accepts that the JHSE will not be held legally responsible for any claims of compensation.
Authors wishing to include figures or text passages that have already been published elsewhere are required to obtain permission from the copyright holder(s) and to include evidence that such permission has been granted when submitting their papers. Any material received without such evidence will be assumed to originate from the authors.
Please include at the end of the acknowledgements a declaration that the experiments comply with the current laws of the country in which they were performed. The editors reserve the right to reject manuscripts that do not comply with the abovementioned requirements. The author(s) will be held responsible for false statements or failure to fulfill the above-mentioned requirements.
This title is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license (CC BY-NC-ND 4.0).
You are free to share, copy and redistribute the material in any medium or format. The licensor cannot revoke these freedoms as long as you follow the license terms under the following terms:
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
NonCommercial — You may not use the material for commercial purposes.
NoDerivatives — If you remix, transform, or build upon the material, you may not distribute the modified material.
No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Notices:
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation.
No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.
Transfer of Copyright
In consideration of JHSE’s publication of the Work, the authors hereby transfer, assign, and otherwise convey all copyright ownership worldwide, in all languages, and in all forms of media now or hereafter known, including electronic media such as CD-ROM, Internet, and Intranet, to JHSE. If JHSE should decide for any reason not to publish an author’s submission to the Work, JHSE shall give prompt notice of its decision to the corresponding author, this agreement shall terminate, and neither the author nor JHSE shall be under any further liability or obligation.
Each author certifies that he or she has no commercial associations (e.g., consultancies, stock ownership, equity interest, patent/licensing arrangements, etc.) that might pose a conflict of interest in connection with the submitted article, except as disclosed on a separate attachment. All funding sources supporting the Work and all institutional or corporate affiliations of the authors are acknowledged in a footnote in the Work.
Each author certifies that his or her institution has approved the protocol for any investigation involving humans or animals and that all experimentation was conducted in conformity with ethical and humane principles of research.
Competing Interests
Biomedical journals typically require authors and reviewers to declare if they have any competing interests with regard to their research.
JHSE require authors to agree to Copyright Notice as part of the submission process.
