Effect of Resistance and Power Training with Walking in Different Directions on the Serum Concentrations of P3NP and CAF, the Lower Body Muscle Strength and Motor Function in Elderly Men
DOI:
https://doi.org/10.14198/jhse.2023.182.14Keywords:
Sport Medicine, Nutrition, Health, N-terminal peptide of type III procollagen (P3NP), C-terminal agrin fragment (CAF), Resistance training, Power training, Walking in four directionsAbstract
Objective(s): Examine the effect of resistance and power training with walking in four directions on the N-terminal peptide of type III procollagen (P3NP), the C-terminal agrin fragment (CAF), the lower body muscle strength and motor function in elderly people. Materials and Methods: Thirty-one elderly men were selected and divided into three groups: control group (C); resistance training (RT) group, and power training (PT) group. RT was performed with 8–10 repetitions during 20–35 seconds of exercise and PT with 8–10 repetitions during 10–13 seconds of exercise. The exercise to which subjects were exposed to, in both resistance and power training groups, consisted in walking in four directions for 12 weeks (two sessions per week). Body mass index (BMI), functional tests, and lower body strength were also measured. The serum levels of P3NP and CAF were evaluated in all groups. Results: Lower body strength (leg press, plantar ankle flexion, knee extension, leg curl) and motor function (chair stand test, timed up and go, 6-minute walking), improved following the exercise, while no effect was found concerning the serum levels of P3NP and CAF. Conclusions: An increase in motor function and lower body strength was observed in both types of exercise programmes discussed in this paper, although the exercise should be done rapidly for a better result.
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Aagaard, P., Simonsen, E., Andersen, J., Magnusson, S., Bojsen‐Møller, F., & Dyhre‐Poulsen, P. (2000). Antagonist muscle coactivation during isokinetic knee extension. Scandinavian journal of medicine & science in sports, 10(2), 58-67. https://doi.org/10.1034/j.1600-0838.2000.010002058.x
Aartolahti, E., Lönnroos, E., Hartikainen, S., & Häkkinen, A. (2019). Long-term strength and balance training in prevention of decline in muscle strength and mobility in older adults. Aging clinical and experimental research, 1-8. https://doi.org/10.1007/s40520-019-01155-0
Arnarson, A., Geirsdottir, O. G., Ramel, A., Briem, K., Jonsson, P., & Thorsdottir, I. (2013). Effects of whey proteins and carbohydrates on the efficacy of resistance training in elderly people: double blind, randomised controlled trial. European journal of clinical nutrition, 67(8), 821. https://doi.org/10.1038/ejcn.2013.40
Arnold, P., & Bautmans, I. (2014). The influence of strength training on muscle activation in elderly persons: a systematic review and meta-analysis. Experimental gerontology, 58, 58-68. https://doi.org/10.1016/j.exger.2014.07.012
Babbs, C., Hunt, L., Haboubi, N., Smith, A., Rowan, B., & Warnes, T. (1988). Type III procollagen peptide: a marker of disease activity and prognosis in primary biliary cirrhosis. The Lancet, 331(8593), 1021-1024. https://doi.org/10.1016/s0140-6736(88)91843-0
Baines, H. L., Turnbull, D. M., & Greaves, L. C. (2014). Human stem cell aging: do mitochondrial DNA mutations have a causal role? Aging Cell, 13(2), 201-205. https://doi.org/10.1111/acel.12199
Bell, K. E., Brook, M. S., Snijders, T., Kumbhare, D., Parise, G., Smith, K., Atherton, P. J., & Phillips, S. M. (2019). Integrated myofibrillar protein synthesis in recovery from unaccustomed and accustomed resistance exercise with and without multi-ingredient supplementation in overweight older men. Frontiers in nutrition, 6, 40. https://doi.org/10.3389/fnut.2019.00040
Bellet, R. N., Adams, L., & Morris, N. R. (2012). The 6-minute walk test in outpatient cardiac rehabilitation: validity, reliability and responsiveness—a systematic review. Physiotherapy, 98(4), 277-286. https://doi.org/10.1016/j.physio.2011.11.003
Bernat, P., Candow, D. G., Grzyb, K., Butchart, S., Schoenfeld, B. J., & Bruno, P. (2019). Effects of high-velocity resistance training and creatine supplementation in untrained healthy aging males. Applied Physiology, Nutrition, and Metabolism(ja). https://doi.org/10.1139/apnm-2019-0066
Berry, S. D., Ramachandran, V. S., Cawthon, P. M., Gona, P., McLean, R. R., Cupples, L. A., & Kiel, D. P. (2013). Procollagen type III N-terminal peptide (P3NP) and lean mass: a cross-sectional study. The Journal of frailty & aging/[editor in chief M. Cesari], 2(3), 129. https://doi.org/10.14283/jfa.2013.19
Bhasin, S., He, E. J., Kawakubo, M., Schroeder, E. T., Yarasheski, K., Opiteck, G. J., Reicin, A., Chen, F., Lam, R., & Tsou, J. A. (2009). N-terminal propeptide of type III procollagen as a biomarker of anabolic response to recombinant human GH and testosterone. The Journal of Clinical Endocrinology & Metabolism, 94(11), 4224-4233. https://doi.org/10.1210/jc.2009-1434
Biolo, A., Rohde, L. E., Goldraich, L. A., Mascarenhas, M., Palombini, D. V., & Clausell, N. (2009). Serum procollagen type III is associated with elevated right-sided filling pressures in stable outpatients with congestive heart failure. Biomarkers, 14(6), 438-442. https://doi.org/10.1080/13547500903098293
Bondoc, I., Cochrane, S. K., Church, T. S., Dahinden, P., Hettwer, S., Hsu, F.-C., Stafford, R. S., Pahor, M., Buford, T. W., & Investigators, L. S. (2015). Effects of a one-year physical activity program on serum C-terminal Agrin Fragment (CAF) concentrations among mobility-limited older adults. The journal of nutrition, health & aging, 19(9), 922-927. https://doi.org/10.1007/s12603-015-0474-3
Breen, L., & Phillips, S. M. (2011). Skeletal muscle protein metabolism in the elderly: Interventions to counteract the'anabolic resistance'of ageing. Nutrition & metabolism, 8(1), 1-11. https://doi.org/10.1186/1743-7075-8-68
Brzycki, M. (1993). Strength testing—predicting a one-rep max from reps-to-fatigue. Journal of Physical Education, Recreation & Dance, 64(1), 88-90. https://doi.org/10.1080/07303084.1993.10606684
Bütikofer, L., Zurlinden, A., Bolliger, M. F., Kunz, B., & Sonderegger, P. (2011). Destabilization of the neuromuscular junction by proteolytic cleavage of agrin results in precocious sarcopenia. The FASEB Journal, 25(12), 4378-4393. https://doi.org/10.1096/fj.11-191262
Calvani, R., Picca, A., Cesari, M., Tosato, M., Marini, F., Manes-Gravina, E., Bernabei, R., Landi, F., & Marzetti, E. (2018). Biomarkers for sarcopenia: reductionism vs. complexity. Current Protein and Peptide Science, 19(7), 639-642. https://doi.org/10.2174/1389203718666170516115422
Chan, P. P., Tou, J. I. S., Mimi, M. T., & Ng, S. S. (2017). Reliability and validity of the timed up and go test with a motor task in people with chronic stroke. Archives of physical medicine and rehabilitation, 98(11), 2213-2220. https://doi.org/10.1016/j.apmr.2017.03.008
Conlon, J. A., Newton, R. U., Tufano, J. J., Peñailillo, L. E., Banyard, H. G., Hopper, A. J., Ridge, A. J., & Haff, G. G. (2017). The efficacy of periodised resistance training on neuromuscular adaptation in older adults. European journal of applied physiology, 117(6), 1181-1194. https://doi.org/10.1007/s00421-017-3605-1
Craig, B., Brown, R., & Everhart, J. (1989). Effects of progressive resistance training on growth hormone and testosterone levels in young and elderly subjects. Mechanisms of ageing and development, 49(2), 159-169. https://doi.org/10.1016/0047-6374(89)90099-7
Deschenes, M. R., Roby, M. A., Eason, M. K., & Harris, M. B. (2010). Remodeling of the neuromuscular junction precedes sarcopenia related alterations in myofibers. Experimental gerontology, 45(5), 389-393. https://doi.org/10.1016/j.exger.2010.03.007
Dieterich, M., & Brandt, T. (2015). The bilateral central vestibular system: its pathways, functions, and disorders. Annals of the New York Academy of Sciences, 1343(1), 10-26. https://doi.org/10.1111/nyas.12585
Díez, J., Laviades, C. n., Mayor, G., Gil, M. a. J. s., & Monreal, I. (1995). Increased serum concentrations of procollagen peptides in essential hypertension: relation to cardiac alterations. Circulation, 91(5), 1450-1456. https://doi.org/10.1161/01.cir.91.5.1450
Drey, M., Sieber, C., Bauer, J., Uter, W., Dahinden, P., Fariello, R., & Vrijbloed, J. (2013). C-terminal Agrin Fragment as a potential marker for sarcopenia caused by degeneration of the neuromuscular junction. Experimental gerontology, 48(1), 76-80. https://doi.org/10.1016/j.exger.2012.05.021
Dufek, J. S., Mercer, J. A., Aldridge, J. M., Melcher, G. G., & Gouws, P.-L. (2009). Effects of Backward Walking on Balance and Lower Extremity Walking Kinematics in Healthy Young and Older Adults. American Society of Biomechanics Annual Meeting, College Park, PA.
Egan, B., & Zierath, J. R. (2013). Exercise metabolism and the molecular regulation of skeletal muscle adaptation. Cell metabolism, 17(2), 162-184. https://doi.org/10.1016/j.cmet.2012.12.012
Erotokritou-Mulligan, I., Bassett, E. E., Kniess, A., Sönksen, P. H., & Holt, R. I. (2007). Validation of the growth hormone (GH)-dependent marker method of detecting GH abuse in sport through the use of independent data sets. Growth hormone & IGF research, 17(5), 416-423. https://doi.org/10.1016/j.ghir.2007.04.013
Erotokritou‐Mulligan, I., Bassett, E. E., Cowan, D. A., Bartlett, C., McHugh, C., Sönksen, P. H., Holt, R. I., & group, G. (2009). Influence of ethnicity on IGF‐I and procollagen III peptide (P‐III‐P) in elite athletes and its effect on the ability to detect GH abuse. Clinical endocrinology, 70(1), 161-168. https://doi.org/10.1111/j.1365-2265.2008.03319.x
Foldvari, M., Clark, M., Laviolette, L. C., Bernstein, M. A., Kaliton, D., Castaneda, C., Pu, C. T., Hausdorff, J. M., Fielding, R. A., & Singh, M. A. F. (2000). Association of muscle power with functional status in community-dwelling elderly women. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 55(4), M192-M199. https://doi.org/10.1093/gerona/55.4.m192
Fragala, M. S., Jajtner, A. R., Beyer, K. S., Townsend, J. R., Emerson, N. S., Scanlon, T. C., Oliveira, L. P., Hoffman, J. R., & Stout, J. R. (2014). Biomarkers of muscle quality: N-terminal propeptide of type III procollagen and C-terminal agrin fragment responses to resistance exercise training in older adults. Journal of cachexia, sarcopenia and muscle, 5(2), 139-148. https://doi.org/10.1007/s13539-013-0120-z
Fragala, M. S., Kenny, A. M., & Kuchel, G. A. (2015). Muscle quality in aging: a multi-dimensional approach to muscle functioning with applications for treatment. Sports medicine, 45(5), 641-658. https://doi.org/10.1007/s40279-015-0305-z
Franzen, P., Haab, D., Mehrabi, M., Binder, M., Plesch, K., Pacher, R., Grimm, M., Pribill, I., Eichler, H.-G., & Glogar, H. D. (1995). Measuring extracellular matrix turnover in the serum of patients with idiopathic or ischemic dilated cardiomyopathy and impact on diagnosis and prognosis. The American journal of cardiology, 75(14), 913-918. https://doi.org/10.1016/s0002-9149(99)80686-9
Gabriel, D. A., Kamen, G., & Frost, G. (2006). Neural adaptations to resistive exercise. Sports medicine, 36(2), 133-149. https://doi.org/10.2165/00007256-200636020-00004
Gomes, M. J., Martinez, P. F., Pagan, L. U., Damatto, R. L., Cezar, M. D. M., Lima, A. R. R., Okoshi, K., & Okoshi, M. P. (2017). Skeletal muscle aging: influence of oxidative stress and physical exercise. Oncotarget, 8(12), 20428. https://doi.org/10.18632/oncotarget.14670
Gouspillou, G., & Hepple, R. T. (2013). Facts and controversies in our understanding of how caloric restriction impacts the mitochondrion. Experimental gerontology, 48(10), 1075-1084. https://doi.org/10.1016/j.exger.2013.03.004
Granacher, U., Muehlbauer, T., & Gruber, M. (2012). A qualitative review of balance and strength performance in healthy older adults: impact for testing and training. Journal of aging research, 2012. https://doi.org/10.1155/2012/708905
Hamilton, D. M., & Haennel, R. (2000). Validity and reliability of the 6-minute walk test in a cardiac rehabilitation population. Journal of Cardiopulmonary Rehabilitation and Prevention, 20(3), 156-164. https://doi.org/10.1097/00008483-200005000-00003
Hortobágyi, T., & DeVita, P. (2006). Mechanisms responsible for the age-associated increase in coactivation of antagonist muscles. Exercise and sport sciences reviews, 34(1), 29-35. https://doi.org/10.1097/00003677-200601000-00007
Izquierdo, M., Hakkinen, K., Ibanez, J., Anton, A., Garrues, M., Ruesta, M., & Gorostiaga, E. M. (2003). Effects of strength training on submaximal and maximal endurance performance capacity in middle-aged and older men. Journal of Strength and Conditioning Research, 17(1), 129-139. https://doi.org/10.1519/00124278-200302000-00021
Jensen, L. T., Hørslev-Petersen, K., Toft, P., Bentsen, K. D., Grande, P., Simonsen, E., & Lorenzen, I. (1990). Serum aminoterminal type III procollagen peptide reflects repair after acute myocardial infarction. Circulation, 81(1), 52-57. https://doi.org/10.1161/01.cir.81.1.52
Kamen, G., & Knight, C. A. (2004). Training-related adaptations in motor unit discharge rate in young and older adults. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 59(12), 1334-1338. https://doi.org/10.1093/gerona/59.12.1334
Kubo, K., Tsunoda, N., Kanehisa, H., & Fukunaga, T. (2004). Activation of agonist and antagonist muscles at different joint angles during maximal isometric efforts. European journal of applied physiology, 91(2), 349-352. https://doi.org/10.1007/s00421-003-1025-x
Larsson, L., Degens, H., Li, M., Salviati, L., Lee, Y. i., Thompson, W., Kirkland, J. L., & Sandri, M. (2018). Sarcopenia: aging-related loss of muscle mass and function. Physiological reviews, 99(1), 427-511. https://doi.org/10.1152/physrev.00061.2017
Lee, W. J., Kim, M., Park, H.-S., Kim, H. S., Jeon, M. J., Oh, K. S., Koh, E. H., Won, J. C., Kim, M.-S., & Oh, G. T. (2006). AMPK activation increases fatty acid oxidation in skeletal muscle by activating PPARα and PGC-1. Biochemical and biophysical research communications, 340(1), 291-295. https://doi.org/10.1016/j.bbrc.2005.12.011
Lelard, T., & Ahmaidi, S. (2015). Effects of physical training on age-related balance and postural control. Neurophysiologie Clinique/Clinical Neurophysiology, 45(4-5), 357-369. https://doi.org/10.1016/j.neucli.2015.09.008
Li, H., Sharma, L. K., Li, Y., Hu, P., Idowu, A., Liu, D., Lu, J., & Bai, Y. (2013). Comparative bioenergetic study of neuronal and muscle mitochondria during aging. Free Radical Biology and Medicine, 63, 30-40. https://doi.org/10.1016/j.freeradbiomed.2013.04.030
Liang, H., Ward, W. F., Jang, Y. C., Bhattacharya, A., Bokov, A. F., Li, Y., Jernigan, A., Richardson, A., & Van Remmen, H. (2011). PGC‐1α protects neurons and alters disease progression in an amyotrophic lateral sclerosis mouse model. Muscle & nerve, 44(6), 947-956. https://doi.org/10.1002/mus.22217
Marcinik, E. J., Potts, J., Schlabach, G., Will, S., Dawson, P., & Hurley, B. (1991). Effects of strength training on lactate threshold and endurance performance. Medicine and science in sports and exercise, 23(6), 739-743. https://doi.org/10.1249/00005768-199106000-00014
Miszko, T. A., Cress, M. E., Slade, J. M., Covey, C. J., Agrawal, S. K., & Doerr, C. E. (2003). Effect of strength and power training on physical function in community-dwelling older adults. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 58(2), M171-M175. https://doi.org/10.1093/gerona/58.2.m171
Morello, R. T., Soh, S.-E., Behm, K., Egan, A., Ayton, D., Hill, K., Flicker, L., Etherton-Beer, C. D., Arendts, G., & Waldron, N. (2019). Multifactorial falls prevention programmes for older adults presenting to the emergency department with a fall: systematic review and meta-analysis. Injury prevention, 25(6), 557-564. https://doi.org/10.1136/injuryprev-2019-043214
Munn, J., Herbert, R. D., Hancock, M. J., & Gandevia, S. C. (2005). Resistance training for strength: effect of number of sets and contraction speed. Medicine and science in sports and exercise, 37(9), 1622. https://doi.org/10.1249/01.mss.0000177583.41245.f8
Nelson, A., Howe, C., Nguyen, T., Leung, K.-C., Trout, G., Seibel, M., Baxter, R., Handelsman, D., Kazlauskas, R., & Ho, K. (2006). Influence of demographic factors and sport type on growth hormone-responsive markers in elite athletes. The Journal of Clinical Endocrinology & Metabolism, 91(11), 4424-4432. https://doi.org/10.1210/jc.2006-0612
Nelson, A. E., Meinhardt, U., Hansen, J. L., Walker, I. H., Stone, G., Howe, C. J., Leung, K.-c., Seibel, M. J., Baxter, R. C., & Handelsman, D. J. (2008). Pharmacodynamics of growth hormone abuse biomarkers and the influence of gender and testosterone: a randomized double-blind placebo-controlled study in young recreational athletes. The Journal of Clinical Endocrinology & Metabolism, 93(6), 2213-2222. https://doi.org/10.1210/jc.2008-0402
Nes, B., Janszky, I., Wisløff, U., Støylen, A., & Karlsen, T. (2013). Age‐predicted maximal heart rate in healthy subjects: The HUNT F itness S tudy. Scandinavian journal of medicine & science in sports, 23(6), 697-704. https://doi.org/10.1111/j.1600-0838.2012.01445.x
Orr, R., De Vos, N. J., Singh, N. A., Ross, D. A., Stavrinos, T. M., & Fiatarone-Singh, M. A. (2006). Power training improves balance in healthy older adults. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 61(1), 78-85. https://doi.org/10.1093/gerona/61.1.78
Perrini, S., Laviola, L., Natalicchio, A., & Giorgino, F. (2005). Associated hormonal declines in aging: DHEAS. J Endocrinol Invest, 28(3 Suppl), 85-93.
Peterka, R. J. (2002). Sensorimotor integration in human postural control. Journal of neurophysiology, 88(3), 1097-1118. https://doi.org/10.1152/jn.2002.88.3.1097
Piasecki, M., Ireland, A., Coulson, J., Stashuk, D. W., Hamilton‐Wright, A., Swiecicka, A., Rutter, M. K., McPhee, J. S., & Jones, D. A. (2016). Motor unit number estimates and neuromuscular transmission in the tibialis anterior of master athletes: evidence that athletic older people are not spared from age‐related motor unit remodeling. Physiological reports, 4(19). https://doi.org/10.14814/phy2.12987
Purath, J., Buchholz, S. W., & Kark, D. L. (2009). Physical fitness assessment of older adults in the primary care setting. Journal of the American Academy of Nurse Practitioners, 21(2), 101-107. https://doi.org/10.1111/j.1745-7599.2008.00391.x
Radaelli, R., Botton, C. E., Wilhelm, E. N., Bottaro, M., Brown, L. E., Lacerda, F., Gaya, A., Moraes, K., Peruzzolo, A., & Pinto, R. S. (2014). Time course of low-and high-volume strength training on neuromuscular adaptations and muscle quality in older women. Age, 36(2), 881-892. https://doi.org/10.1007/s11357-013-9611-2
Ramírez-Campillo, R., Castillo, A., Carlos, I., Campos-Jara, C., Andrade, D. C., Álvarez, C., Martínez, C., Castro-Sepúlveda, M., Pereira, A., & Marques, M. C. (2014). High-speed resistance training is more effective than low-speed resistance training to increase functional capacity and muscle performance in older women. Experimental gerontology, 58, 51-57. https://doi.org/10.1016/j.exger.2014.07.001
Reid, K. F., Martin, K. I., Doros, G., Clark, D. J., Hau, C., Patten, C., Phillips, E. M., Frontera, W. R., & Fielding, R. A. (2014). Comparative effects of light or heavy resistance power training for improving lower extremity power and physical performance in mobility-limited older adults. Journals of Gerontology Series A: Biomedical Sciences and Medical Sciences, 70(3), 374-380. https://doi.org/10.1093/gerona/glu156
Santanasto, A. J., Cvejkus, R. K., Wojczynski, M. K., Marron, M. M., Schupf, N., Christensen, K., Thyagarajan, B., & Zmuda, J. M. (2020). Circulating Procollagen Type III N-Terminal Peptide and Physical Function in Adults from the Long Life Family Study. The Journals of Gerontology: Series A. https://doi.org/10.1093/gerona/glaa197
Semmler, J. G. (2002). Motor unit synchronization and neuromuscular performance. Exercise and sport sciences reviews, 30(1), 8-14. https://doi.org/10.1097/00003677-200201000-00003
Simons, R., & Andel, R. (2006). The effects of resistance training and walking on functional fitness in advanced old age. Journal of aging and health, 18(1), 91-105. https://doi.org/10.1177/0898264305281102
Steadman, J., Donaldson, N., & Kalra, L. (2003). A randomized controlled trial of an enhanced balance training program to improve mobility and reduce falls in elderly patients. Journal of the American Geriatrics Society, 51(6), 847-852. https://doi.org/10.1046/j.1365-2389.2003.51268.x
Stephan, A., Mateos, J. M., Kozlov, S. V., Cinelli, P., Kistler, A. D., Hettwer, S., Rülicke, T., Streit, P., Kunz, B., & Sonderegger, P. (2008). Neurotrypsin cleaves agrin locally at the synapse. The FASEB Journal, 22(6), 1861-1873. https://doi.org/10.1096/fj.07-100008
Straight, C. R., Lindheimer, J. B., Brady, A. O., Dishman, R. K., & Evans, E. M. (2016). Effects of resistance training on lower-extremity muscle power in middle-aged and older adults: a systematic review and meta-analysis of randomized controlled trials. Sports Medicine, 46(3), 353-364. https://doi.org/10.1007/s40279-015-0418-4
Sugimoto, M., Masutani, S., Seki, M., Kajino, H., Fujieda, K., & Senzaki, H. (2009). High serum levels of procollagen type III N-terminal amino peptide in patients with congenital heart disease. Heart, 95(24), 2023-2028. https://doi.org/10.1136/hrt.2009.170241
Tudoraşcu, I., Sfredel, V., Riza, A. L., Dănciulescu Miulescu, R., Ianoşi, S. L., & Dănoiu, S. (2014). Motor unit changes in normal aging: a brief review. Rom J Morphol Embryol, 55(4), 1295-1301.
Ylitalo, K. R., Karvonen-Gutierrez, C. A., Sternfeld, B., & Pettee Gabriel, K. (2021). Association of physical activity and physical functioning phenotypes with fall risk among women. Journal of aging and health. https://doi.org/10.1177/0898264320988405
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