Effects of acute caffeine intake on sex hormones response and repetitions to failure in resistance-trained females during early follicular phase


  • Mohammad AbuMoh'd Al-Ahliyya Amman University, Jordan




Sport medicine, Health, Muscular endurance, Menstruation, Pituitary, Hypothalamus, Oral contraceptive pills


This study investigated the acute effects of caffeine intake on sex hormones (follicle-stimulating hormone, luteinizing hormone, prolactin, oestradiol, and progesterone) following resistance exercise performance during the early follicular phase. In addition, the total number of failed repetitions was determined. Ten resistance-trained females performed two consecutive trials (48 h apart). Participants were randomly assigned to receive either caffeine (4 mg/kg) 1 h before exercise or a placebo, using a double-blind crossover design. Each trial included the following resistance exercises: chest press, lat pulldown, triceps pushdown, and rowing torso. Each exercise was performed in three sets of 10 repetitions at 60% of 1-RM, with a 90 s recovery interval, followed by repetitions to failure at the same intensity during the fourth set. Two-minute breaks were allocated between each exercise’s third and fourth sets of and between exercises. Blood samples were collected from each participant 1 h after the completion of each trial. Data revealed no statistical difference (p ˃ .05) in ergogenic response to caffeine on sex hormones during the early follicular phase between trials. However, serum prolactin level significantly decreased (p = .039) after caffeine intake compared to the placebo. The overall repetitions to failure were significantly higher (p = .023) in the caffeine trial than in the placebo. In conclusion, caffeine intake (4 mg/kg) 1 h before multiple resistance exercises had no effect on sex hormone responses during the early follicular phase, except for prolactin. However, the overall repetitions until failure were higher following caffeine intake.


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Ansdell, P., Brownstein, C. G., Škarabot, J., Hicks, K. M., Simoes, D. C. M., Thomas, K., Howatson, G., Hunter, S. K., & Goodall, S. (2019). Menstrual cycle-associated modulations in neuromuscular function and fatigability of the knee extensors in eumenorrheic women. Journal of Applied Physiology, 126(6), 1701-1712. https://doi.org/10.1152/japplphysiol.01041.2018

Bowtell, J. L., Mohr, M., Fulford, J., Jackman, S. R., Ermidis, G., Krustrup, P., & Mileva, K. N. (2018). Improved Exercise Tolerance with Caffeine Is Associated with Modulation of both Peripheral and Central Neural Processes in Human Participants. Frontiers in nutrition, 5, 6. https://doi.org/10.3389/fnut.2018.00006

Dasa, M. S., Kristoffersen, M., Ersvær, E., Bovim, L. P., Bjørkhaug, L., Moe-Nilssen, R., Sagen, J. V., & Haukenes, I. (2021). The Female Menstrual Cycles Effect on Strength and Power Parameters in High-Level Female Team Athletes. Frontiers in physiology, 12, 600668. https://doi.org/10.3389/fphys.2021.600668

Davis, J.-K., Green, J. M., & Laurent, C. M. (2012). Effects of Caffeine on Resistance Training Performance on Repetitions to Failure. Journal of Caffeine Research, 2(1), 31-37. https://doi.org/10.1089/jcr.2012.0005

Del Coso, J., Muñoz, G., & Muñoz-Guerra, J. (2011). Prevalence of caffeine use in elite athletes following its removal from the World Anti-Doping Agency list of banned substances. Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme, 36(4), 555-561. https://doi.org/10.1139/h11-052

Dodd, F. L., Kennedy, D. O., Riby, L. M., & Haskell-Ramsay, C. F. (2015). A double-blind, placebo-controlled study evaluating the effects of caffeine and L-theanine both alone and in combination on cerebral blood flow, cognition and mood. Psychopharmacology, 232(14), 2563-2576. https://doi.org/10.1007/s00213-015-3895-0

Duncan, M. J., Stanley, M., Parkhouse, N., Cook, K., & Smith, M. (2013). Acute caffeine ingestion enhances strength performance and reduces perceived exertion and muscle pain perception during resistance exercise. European Journal of Sport Science, 13(4), 392-399. https://doi.org/10.1080/17461391.2011.635811

Filip-Stachnik, A., Wilk, M., Krzysztofik, M., Lulińska, E., Tufano, J. J., Zajac, A., Stastny, P., & Del Coso, J. (2021). The effects of different doses of caffeine on maximal strength and strength-endurance in women habituated to caffeine. Journal of the International Society of Sports Nutrition, 18(1), 25. https://doi.org/10.1186/s12970-021-00421-9

Goldstein, E., Jacobs, P. L., Whitehurst, M., Penhollow, T., & Antonio, J. (2010). Caffeine enhances upper body strength in resistance-trained women. Journal of the International Society of Sports Nutrition, 7(1), 18. https://doi.org/10.1186/1550-2783-7-18

Graham T. E. (2001). Caffeine and exercise: metabolism, endurance and performance. Sports medicine (Auckland, N.Z.), 31(11), 785-807. https://doi.org/10.2165/00007256-200131110-00002

Green, J., Wickwire, P., McLester, J., Gendle, S., Hudson, G., Pritchett, R., & Laurent, M. (2007). Effects of Caffeine on Repetitions to Failure and Ratings of Perceived Exertion During Resistance Training. International Journal of Sports Physiology and Performance, 2, 250-259. https://doi.org/10.1123/ijspp.2.3.250

Grgic, J., & Mikulic, P. (2017). Caffeine ingestion acutely enhances muscular strength and power but not muscular endurance in resistance-trained men. European journal of sport science, 17(8), 1029-1036. https://doi.org/10.1080/17461391.2017.1330362

Grgic, J., & Mikulic, P. (2021). Acute effects of caffeine supplementation on resistance exercise, jumping, and Wingate performance: no influence of habitual caffeine intake. European Journal of Sport Science, 21(8), 1165-1175. https://doi.org/10.1080/17461391.2020.1817155

Heavens, K. R., Szivak, T. K., Hooper, D. R., Dunn-Lewis, C., Comstock, B. A., Flanagan, S. D., Looney, D. P., Kupchak, B. R., Maresh, C. M., Volek, J. S., & Kraemer, W. J. (2014). The Effects of High Intensity Short Rest Resistance Exercise on Muscle Damage Markers in Men and Women. The Journal of Strength & Conditioning Research, 28(4). https://doi.org/10.1097/JSC.0000000000000236

Klentrou, P., & Plyley, M. (2003). Onset of puberty, menstrual frequency, and body fat in elite rhythmic gymnasts compared with normal controls. British journal of sports medicine, 37(6), 490-494. https://doi.org/10.1136/bjsm.37.6.490

Lara, B., Gutiérrez Hellín, J., Ruíz-Moreno, C., Romero-Moraleda, B., & Del Coso, J. (2020). Acute caffeine intake increases performance in the 15-s Wingate test during the menstrual cycle. British journal of clinical pharmacology, 86(4), 745-752. https://doi.org/10.1111/bcp.14175

Martins, G. L., Guilherme, J. P. L. F., Ferreira, L. H. B., de Souza-Junior, T. P., & Lancha, A. H., Jr (2020). Caffeine and Exercise Performance: Possible Directions for Definitive Findings. Frontiers in sports and active living, 2, 574854. https://doi.org/10.3389/fspor.2020.574854

Norum, M., Risvang, L. C., Bjørnsen, T., Dimitriou, L., Rønning, P. O., Bjørgen, M., & Raastad, T. (2020). Caffeine increases strength and power performance in resistance-trained females during early follicular phase. Scandinavian journal of medicine & science in sports, 30(11), 2116-2129. https://doi.org/10.1111/sms.13776

Polito, M. D., Grandolfi, K., & de Souza, D. B. (2019). Caffeine and resistance exercise: the effects of two caffeine doses and the influence of individual perception of caffeine. European journal of sport science, 19(10), 1342-1348. https://doi.org/10.1080/17461391.2019.1596166

Raastad, T., Bjøro, T., & Hallén, J. (2000). Hormonal responses to high- and moderate-intensity strength exercise. European journal of applied physiology, 82(1-2), 121-128. https://doi.org/10.1007/s004210050661

Romero-Moraleda, B., Del Coso, J., Gutiérrez-Hellín, J., & Lara, B. (2019). The Effect of Caffeine on the Velocity of Half-Squat Exercise during the Menstrual Cycle: A Randomized Controlled Trial. Nutrients, 11(11), 2662. https://doi.org/10.3390/nu11112662

Ruiz-Fernández, I., Valadés, D., Dominguez, R., Ferragut, C., & Pérez-López, A. (2023). Load and muscle group size influence the ergogenic effect of acute caffeine intake in muscular strength, power and endurance. European journal of nutrition, 62(4), 1783-1794. https://doi.org/10.1007/s00394-023-03109-9

Salinero, J. J., Lara, B., Jiménez-Ormeño, E., Romero-Moraleda, B., Giráldez-Costas, V., Baltazar-Martins, G., & Del Coso, J. (2019). More Research Is Necessary to Establish the Ergogenic Effect of Caffeine in Female Athletes. Nutrients, 11(7), 1600. https://doi.org/10.3390/nu11071600

Seyed Saadat, S. N., Mohammadghasemi, F., Ebrahimi, H., Rafati Sajedi, H., & Chatrnour, G. (2016). Ovarian and uterine alterations following forced swimming: An immunohistochemical study. International journal of reproductive biomedicine, 14(10), 629-636. https://doi.org/10.29252/ijrm.14.10.629

Smilios, I., Tsoukos, P., Zafeiridis, A., Spassis, A., & Tokmakidis, S. P. (2014). Hormonal responses after resistance exercise performed with maximum and submaximum movement velocities. Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme, 39(3), 351-357. https://doi.org/10.1139/apnm-2013-0147

Tarnopolsky M. A. (2010). Caffeine and creatine use in sport. Annals of nutrition & metabolism, 57 Suppl 2, 1-8. https://doi.org/10.1159/000322696

Tinsley, G. M., Urbina, S., Mullins, J., Outlaw, J., Hayward, S., Stone, M., Foster, C., Wilborn, C., & Taylor, L. (2017). Influence of A Thermogenic Dietary Supplement on Safety Markers, Body Composition, Energy Expenditure, Muscular Performance and Hormone Concentrations: A Randomized, Placebo-Controlled, Double-Blind Trial. Journal of Sports Science & Medicine, 16(4), 459-467.

Tortora, J., Gerard, and Derrickson, Bryan. (2017). Principles of Anatomy and Physiology (15th edition). WILEY.

Wilk, M., Krzysztofik, M., Maszczyk, A., Chycki, J., & Zajac, A. (2019). The acute effects of caffeine intake on time under tension and power generated during the bench press movement. Journal of the International Society of Sports Nutrition, 16(1), 8. https://doi.org/10.1186/s12970-019-0275-x

Wu, B.-H., & Lin, J.-C. (2010). Caffeine attenuates acute growth hormone response to a single bout of resistance exercise. Journal of Sports Science & Medicine, 9(2), 262-269.

Effects of acute caffeine intake on sex hormones response and repetitions to failure in resistance-trained females during early follicular phase



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How to Cite

AbuMoh’d, M. (2023). Effects of acute caffeine intake on sex hormones response and repetitions to failure in resistance-trained females during early follicular phase. Journal of Human Sport and Exercise, 19(1), 183–192. https://doi.org/10.14198/jhse.2024.191.16



Sport Medicine, Nutrition & Health