Journal of Human Sport and Exercise

Effects of a carbohydrate and a carbohydrate and casein protein beverages on recovery and performance of endurance cycling capacity

Mar Cepero, F. J. Rojas, A. Geerlings, J. C. de la Cruz, S. Romero, J. J. Boza



The main aim of this study was to determine if short-term post exercise recovery, cycling performance and muscle damage were altered when consuming a carbohydrate-only beverage (CHO, 7% carbohydrate) versus a carbohydrate and casein protein beverage (CHO+P, 7% carbohydrate and 4% protein). Fifteen male cyclists (VO2peak= 63.4±9.6 ml·kg-1·min-1) performed two trials using a randomly counterbalanced, double-blind design. In each trial one liter of one of the test drinks was consumed in fasting conditions after 1 hour ride at 75% VO2peak determined by Arts and Kuipers´ regression equation .After a two hours recovery period the cyclists rode 20 km at the rider's maximum speed for this distance. The results showed no significant differences in the 20-km ride when consuming the CHO (1770±210 s) or the CHO+P drink (1819±185 s). Post-exercise creatine kinase (CK) was not significantly different between treatments. However, serum insulin concentrations were higher during recovery when CHO+P beverage was consumed. Glucagon and lactic acid levels increased more on the CHO than on the CHO+P treatment at the end of the 20 km test. Within the context of this experimental design, the CHO+P drink showed different physiological effects than the CHO drink, so that the CHO+P drink can be recommended for improving recuperation from intensive exercise. Although this was not reflected in post-recovery exercise performance in this 20 km test, a harder or longer test may be more affected by the physiological parameters.


Ergogenic supplement; Casein protein; Recovery; Cycling performance


Arts F.J., Kuipers H. The relation between power output (w), oxygen uptake (Vo2max) and heart rate in male athletes. International Journal of Sports Medicine. 1994; 5(15):228-231.

Betts J., Williams C., Duffy K., Gunner F. The influence of carbohydrate and protein ingestion during recovery from prolonged exercise on subsequent endurance performance. Journal of Sport Sciences. 2007; 25(13):1449-1460.

Bloomer R.J., Goldfarb A.H. Can nutritional supplements reduce exercise-induced skeletal muscle damage? Strength Cond. 2003; 25:30-37.

Colombani P.C., Kovacs C., Frey-Rindova P. Metabolic effects of a protein supplemented carbohydrate drink in marathon runners. Int. J. Sports Nutr. 1999; 9:181-201.

Ivy J.L., Res P.T., Sprague R.C., Widzer M.O. Effect of a carbohydrate-protein supplement on endurance performance during exercise of varying intensity. Int. J. Sport Nutr. Exerc. Metab. 2003; 13 :388-401.

Jentjens R., Jeukendrup A.E. Determinants of post-exercise glycogen synthesis during short-term recovery. Sports Med. 2003; 33(2):117-144.

Jentjens R.L., Achten J., Jeukendrup A.E. High oxidation rates from combined carbohydrates ingested during exercise. Med. Sci. Sports Exerc. 2004; 36(9):1551-1558.

Jeukendrup A., Saris W.H., Brouns F., Kenster A.D. A new validated endurance performance test. Medicine and Science in Sports and Exercise. 1996; 28:266 – 270.

Jeukendrup A.E., Jentjens R. Oxidation of CHO feedings during prolonged exercise: current thoughts, guidelines, and directions for future research. Sports Med. 2000; 29:407-424.

Koopman R., Pannemans L.E., Jeukendrup A.E. Combined ingestion of protein and carbohydrate improves protein balance during ultra-endurance exercise. Am. J. Physiol. Endocrinol. Metab. 2004; 287:E712-E720.

Lemon P.W.R. Effects of exercise on dietary protein requirements. Int. J. Sport Nutr. 1998; 8:426-447.

Lipsey M.W. Design Sensitivity: Statistical Power for Experimental Research. Newbury Park, CA: Sage; 1990. p. 88-92, 133-136.

Luden N.D., Saunders M.J., Todd M.K. Post-exercise carbohydrate-protein-antioxidant ingestion decreases CK and muscle soreness in cross-country runners. Int. J. Sports Nutr. Exerc. Metab. 2007; 17:109-122.

Millard-Stafford M., Warren G., Thomas L., Doyle J., Snow T., Hitchcock K. Recovery from run training: efficacy of a carbohydrate-protein beverage? Int. J. Sport Nutr. Exerc. Metab. 2005; 15:610-624.

Niles E.S., Lachowetz T., Garfi J. Carbohydrate-protein drink improves time to exhaustion after recovery from endurance exercise. J. Exerc. Physiol. Online. 2001; 4:45-52.

Osterberg K.L., Zachwieja J.J., Smith J.W. Carbohydrate and carbohydrate + protein for cycling time-trial performance. Journal of Sports Sciences, 2008; 26(3):227-233.

Romano-Ely B.C., Todd M.K., Saunders M.J., St Laurent T.G. Effects of an isocaloric carbohydrate-protein-antioxidant drink on cycling performance. Med. Sci. Sports Exerc. 2006; 38:1608-1616.

Saunders M.J., Kane M.D., Todd M.K. Effects of a carbohydrate-protein beverage on cycling endurance and muscle damage. Med. Sci. Sports Exerc. 2004; 36:1233-1238.

Saunders M.J., Luden N.D., Herrick J.E. Consumption of an oral carbohydrateprotein gel improves cycling endurance and prevents post-exercise muscle damage. J. Strength Cond. Res. 2007; 21(3):678-685.

Saunders M.J., Luden N.D., Pratt C.A., Moore R.W. Carbohydrate and protein hydrolysate beverage improves late-race cycling performance and prevents post-exercise muscle damage. J. Int. Soc. Sports Nutr. 2006; 3(1): S20.

St Laurent T.G., Todd M.K., Saunders M.J., Valentine R.J., Flohr J.A. Carbohydrate- protein beverage improves muscle damage and function versus isocarbohydrate and isocaloric carbohydrate-only beverages. Med. Sci. Sports Exerc. 2006; 38(5): S340.

Van Essen M., Gibala M.J. Failure of protein to improve time trial performance when added to a sports drink. Med. Sci. Sports Exerc. 2006; 38(8):1476-1483.

Van Hall G., Maclean D.A., Saltin B., Wagenmakers A.J. Mechanisms of activation of muscle branched-chain alpha-keto acid dehydrogenase during exercise in man. J. Physiol. 1996; 494(3):899-905.

Van Loon L.J.C. Application of Protein or Protein Hydrolysates to Improve Postexercise Recovery. International Journal of Sport Nutrition and Exercise Metabolism. 2007; 17: S104-S117.

Van Loon L.J., Kruijshoop M., Verhagen H., Saris W.H., Wagenmakers A.J. Ingestion of protein hydrolysate and amino acid-carbohydrate mixtures increases postexercise plasma insulin responses in men. J. Nutr. 2000; 130:2508-2513.

Williams M.B., Raven P.B., Fogt D.L., Ivy J.L. Effects of recovery beverages on glycogen restoration and endurance exercise performance. J. Strength Cond. Res. 2003; 17:12-19.


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