Preventive effects of garlic and lemon extract combined with aerobic exercise on blood metabolic parameters and liver enzymes



Physical activity, Lipid profile, Hypercholesterolemia, Fatty liver, Cardiovascular disease, Wistar rats


Purpose: The aim of this study was to evaluate the effects of aerobic exercise in combination with garlic and lemon on the lipid profile, metabolic parameters, and liver enzymes of obese male rats. Design: Sixty-four male Wistar rats were divided into eight equal groups consisting of 1) control following no treatment (n = 8); 2) hypercaloric fatty-food-based diet (n = 8); 3) aerobic exercise (AE, n = 8); 4) garlic intake (G, n = 8); 5) aerobic exercise with garlic intake (AEG, n = 8); 6) lemon intake (L, n = 8); 7) garlic and lemon (GL, n = 8); 8) garlic, lemon and aerobic exercise (GLAE, n = 8). After six weeks of intervention, blood samples were taken to obtain cholesterol, triglycerides (TG), high-density lipoprotein cholesterol (HDL), low-density lipoprotein cholesterol (LDL), and glucose. C-reactive protein (CRP), alanine aminotransferase (GPT), aspartate aminotransferase (GOT), alkaline phosphatase (ALP), creatinine (Cr), urea and uric acid (UA) were also measured. A one-way ANOVA or the Kruskal Wallis tests for the non-normally distributed variables, with post-hoc pairwise comparisons were conducted to assess differences between groups. Results: All the intervention groups obtained significantly better values compared to the hypercaloric group, although being also fed with a hypercaloric diet. In certain parameters, the intervention groups obtained equal or even better results than the control healthy group. The combination of aerobic exercise with the intake of garlic and lemon showed slightly non-significant better results. Conclusion: Aerobic exercise combined with the intake of garlic and lemon juice may influence the lipid profile, liver enzymes, and other blood parameters associated with cardiovascular disease.


The authors gratefully acknowledge the cooperation of the Shahrekord Medicinal Plants Research Center, which supported and funded this project.


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Ali M., Al Qattan K. K., Al Enezi F., Khanafer R. M., & Mustafa T. (2000). Effect of allicin from garlic powder on serum lipids and blood pressure in rats fed with a high cholesterol diet. Prostaglandins Leukot Essent Fatty Acids, 62(4), 253-259.

Ascensao, A., Rebelo, A., Oliveira, E., Marques, F., Pereira, L., & Magalhaes, J. (2008). Biochemical impact of a soccer match-analysis of oxidative stress and muscle damage markers throughout recovery. Clinical Biochemistry, 41(10-11), 841-851.

Aslani, N., Entezari, M., Maghsoudi, Z., & Askari, G. (2015). Effect of garlic and lemon juice mixture on fasting blood sugar, diastolic and systolic blood pressure and body mass index in people with hyperlipidemia. Journal of Isfahan Medical School, 32, 320, 2491-2500.

Aslani, N., Entezari, M. H., Askari, G., Maghsoudi, Z., & Maracy, M. R. (2016). Effect of garlic and lemon juice mixture on lipid profile and some cardiovascular risk factors in people 30-60 years old with moderate hyperlipidaemia: A randomized clinical trial. International Journal of Preventive Medicne, 7, 95.

Aucott, L., Poobalan, A., Smith, W. C., Avenell, A., Jung, R., Broom, J., & Grant, A. M. (2004). Weight loss in obese diabetic and non-diabetic individuals and long-term diabetes outcomes - a systematic review. Diabetes Obesity and Metabolism, 6, 85-94.

Banerjee, S. K., & Maulik, S. K. (2002). Effect of garlic on cardiovascular disorders: a review. Nutrition Journal, 1(4).

Batsis, J. A., & Lopez-Jimenez, F. (2010).Cardiovascular risk assessment - From individual risk prediction to estimation of global risk and change in risk in the population. BMC Med;8:29.

Berman, A. Y., Motechin, R. A., Wiesenfeld, M. Y., & Holz, M. K. (2017). The therapeutic potential of resveratrol: a review of clinical trials. NPJ Precision Oncology, 35.

Bhavsar, S. K., Joshi, P., Shah, M. B., & Santani, D. D. (2007). Investigation into hepatoprotective activity of Citrus limon. Pharmaceutical Biology, 45(4), 303-311.

Budoff, M. J., Ahmadi, N., Gul, K. M., Liu, S. T., Flores, F. R., Tiano, J., et al. (2009). Aged garlic extract supplemented with B vitamins, folic acid and L arginine retards the progression of subclinical atherosclerosis: a randomized clinical trial. Preventive Medicine, 49(2,3),101-107.

Dhawan, V., & Jain S. (2004). Effect of garlic supplementation on oxidized low-density lipoproteins and lipid peroxidation in patients of essential hypertension. Molecular and Cellular Biochemistry 266, 109-115.

Durak, I., Kavutcu, M., Aytaç, B., Avci, A., Devrim, E., Ozbek, H., & Öztürka, H. S. (2004). Effects of garlic extract consumption on blood lipid and oxidant/antioxidant parameters in humans with high blood cholesterol. The Journal of Nutritional Biochemistry, 15(6), 373.

Ebrahimi, T., Behdad, B., Abbasi, M. A., Rabati, R. G., Fayyaz, A. F., Behnod, V., & Asgari, A. (2015). High doses of garlic extract significantly attenuated the ratio of serum LDL to HDL level in rat-fed with hypercholesterolemia diet. Diagnostic Pathology, 10(1), 74.

El-Sabban, F. (2009). Garlic as an antithrombotic and antiplatelet aggregation agent. Journal of Chinese Clinical Medicine, 4(5), 288-294.

Folsom, A., Arnett, D., Hutchinson, R., Liao, F., Clegg, L. X., & Cooper, L. S. (1997). Physical activity and incidence of coronary heart disease in middle-aged women and men. Medicine & Science in Sports &Exercise; 29(7), 901-907.

Gorinsteina, S., Leontowiczb, M., Leontowiczb, H., Najmanb, K., Namiesnikc, J., Park, Y. S., Jung, S., Kang, S., & Trakhtenberg, S. (2006). Supplementation of garlic lowers lipids and increases antioxidant capacity in plasma of rats. Nutrition Research, 26(7), 362-368.

Hsu, C. C., Lin, C. C., Liao, T. S., Yin, M. C. (2006). Protective effect of s-allyl cysteine and s-propylcysteine on acetaminophen-induced hepatotoxicity in mice. Food and Chemical Toxicology, 44(3), 393-397.

Kelley, G. A., Kelley, K. S., & Pate, R. R. (2019). Exercise and adiposity in overweight and obese children and adolescents: a systematic review with network meta-analysis of randomised trials. BMJ Open, 9(11), e031220.

Lanzotti, V. (2006). The analysis of onion and garlic. Journal of Chromatography A, 1112(1-2), 3-22.

Marseglia, L., Manti, S., D'Angelo, G., Nicotera, A., Parisi, E., Di Rosa, G., Gitto, E., & Arrigo, T. (2014). Oxidative stress in obesity: a critical component in human diseases. International journal of molecular sciences, 16(1), 378-400.

Matsuura, H. (2001). Saponins in garlic as modifiers of the risk of cardiovascular disease. The Journal of Nutrition, 131(3),1000S-1005S.

Minato, K., Miyake, Y., Fukumoto, S., Yamamoto, K., Kato, Y., Shimomura, Y., Osawa, T. (2003). Lemon flavonoid, eriocitrin, suppresses exercise-induced oxidative damage in rat liver. Life Sci. 21; 72(14):1609-16.

Mobasseri, M., Fakhrzadeh, H., Pourebrahim, R., Nouri, M., Shoushtarizade, P., & Pajuhi, M. (2003). Evaluation of lipid abnormalities in urban population 25-64 years-old in Tehran University of Medical Sciences (population lab region). Iranian Journal of Diabetes and Lipid Disorders, 3(1), 53-62. [In Persian].

Mohamed, M. S., Abdel-Kader, M. M., & Kassem, S. S. (2011). Effect of dietary garlic and onion on liver and tibial mineral concentrations in omega-3 fatty acids rich oil fed rats. Agriculture and Biology Journal of North America, 2(5), 745-51.

Mohammadi, A., & Oshaghi, EA. (2014). Effect of garlic on lipid profile and expression of LXR alpha in intestine and liver of hypercholesterolemic mice. Journal of Diabetes & Metabolic Disorders, 13(20).

Namjoo, A., Heidarian, E., Rafieian-Kopaei, M., & Jafarian-Dehkordi, M. (2013). Effect of chronic oral administration of garlic aqueous extract on tissue changes, some blood and enzymatical parameters in male rats. Journal of Shahrekord University of Medical Science, 15(1), 103-113.

Nichols, L. A., Jackson, D. E., Manthey, J. A., Shukla, S. D., & Holland, L. J. (2011). Citrus flavonoids repress the mRNA for stearoyl-CoA desaturase, a key enzyme in lipid synthesis and obesity control, in rat primary hepatocytes. Lipids in Health and Disease, 10(1), 36.

Rahman, K. (2003). Garlic and aging: new insights into an old remedy. Ageing Research Reviews, 2(1), 39-56.

Ried, K., Frank, O. R., Stocks, N. P., Fakler, P., & Sullivan, T. (2008). Effect of garlic on blood pressure: A systematic review and meta-analysis. BMC Cardiovascular Disorders, 8(1), 13.

Shamsoddini, A., Sobhani, V., Ghamar Chehreh, M. E., Alavian, S. M., & Zaree, A. (2015). Effect of aerobic and resistance exercise training on liver enzymes and hepatic fat in iranian men with nonalcoholic fatty liver disease. Hepatitis Monthly, 15(10), e31434.

Shi, K. Q., Fan, Y. C., Liu, W. Y., Li, L. F., Chen, Y. P., & Zheng, M. H. (2012). Traditional Chinese medicines benefit to nonalcoholic fatty liver disease: a systematic review and meta-analysis. Molecular Biology Reports, 39, 9715-9722.

Sohn, C. W., Kim, H., You, B. R., Kim, M. J., Kim, H. J., Lee, J. Y, & Kim, M. R. (2012). High temperature- and high pressure-processed garlic improves lipid profiles in rats fed high cholesterol diets. Journal of Medicinal Food, 15(5), 435-440.

Torkamaneh, S., Sharifi, GH., & Rafieian, M. (2016). The comparison between effects of Berberis vulgaris Extract and aerobic exercise on none-alcoholic fatty liver in male rat. Der Pharma Chemica, 8(1), 244-247.

Turner, B., Mølgaard, C., & Marckmann, P. (2004). Effect of garlic (Allium sativum) powder tablets on serum lipids, blood pressure and arterial stiffness in normo lipidaemic volunteers: a randomised, double blind, placebo-controlled trial. British Journal of Nutrition, 92, 701-706.

Warshafsky, S., Kamer, R. S., & Sivak, S. L. (1993). Effect of garlic on total serum cholesterol. A meta-analysis. Annals of Internal Medicine,119(7 Pt1), 599-605.

Williamson, J., & Davison, G. (2020). Targeted antioxidants in exercise-induced mitochondrial oxidative stress: emphasis on DNA damage. Antioxidants, 9(11), 1142.

Wood, P. D., Stefanick, M. L., Williams, P. T., & Haskell, W. L. (1991). The effects on plasma lipoproteins of a prudent weight-reducing diet, with or without exercise, in overweight men and women. The New England Journal of Medicine, 325, 461-466.

Xu, Y., Guo, W., Zhang, C., Chen, F., Tan, H. Y., Li, S., Wang, N., & Feng, Y. (2020). Herbal medicine in the treatment of non-alcoholic fatty liver diseases-efficacy, action mechanism, and clinical application. Frontiers in Pharmacology, 11, 601.

Yang, Y., Chan, S. W., Hu, M., Walden, R., & Tomlinson, B. (2011). Effects of some common food constituents on cardiovascular disease. ISRN Cardiology, 2011, 397136.

Yoon, G. (2006). Effect of garlic supplement and exercise on plasma lipid and antioxidant enzyme system in rat. The Korean Journal of Nutrition, 39(1): 3-1.


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How to Cite

Torkamaneh, S., Gene-Morales, J., Flandez, J., Yadav, M., Sidiq, M., Rafieian-Kopaei, M., & Colado, J. C. (2021). Preventive effects of garlic and lemon extract combined with aerobic exercise on blood metabolic parameters and liver enzymes. Journal of Human Sport and Exercise, 16(2proc), S640-S650. Retrieved from

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