The Orexin-A serum levels are strongly modulated by physical activity intervention in diabetes mellitus patients


  • Rita Polito University of Foggia, Italy
  • Vincenzo Cristian Francavilla Kore University of Enna, Italy
  • Antonio Ambrosi University of Foggia, Italy
  • Nicola Tartaglia University of Foggia, Italy
  • Domenico Tafuri University of Naples "Parthenope",
  • Marcellino Monda University of Campania Luigi Vanvitelli, Italy
  • Antonietta Messina University of Campania Luigi Vanvitelli, Italy
  • Francesco Sessa University of Foggia, Italy
  • Girolamo Di Maio University of Campania Luigi Vanvitelli, Italy
  • Alberto Ametta University of Foggia, Italy
  • Alessia Scarinci University of Bari, Italy
  • Vincenzo Monda University of Campania Luigi Vanvitelli, Italy
  • Giovanni Messina University of Foggia, Italy



Type 2 diabetes mellitus (T2DM), Orexin-A, TNF-a, IL-8, IL-10, Physical activity


The Orexin-A (hypocretin-1) is a neuropeptide secreted by neurons in the lateral hypothalamus. This protein regulates physiological and behavioural processes that have an essential impact on energy balance and metabolic status, physical activity, blood glucose levels, and food intake. Furthermore, that orexin-A regulates insulin sensitivity, energy expenditure and metabolic rate and is involved in immune processes and then regulate inflammatory response, with an anti-inflammatory action. Diabetes mellitus (T2DM) is a worldwide health problem associated with obesity and sedentary lifestyle. High glycaemic levels and lipid serum profile, low col-HDL, or hypertension and increased body mass index (BMI) are significantly associated with increased T2DM risk and with increased cardiovascular mortality and morbidity in T2DM patients. For these reasons the aim of this study is to evaluate the biochemical and anthropometric parameters, orexin-A levels by ELISA test and western blotting analysis, and inflammatory cytokines levels such as TNF-a, IL-8 and IL-10 by ELISA test in subjects affected by diabetes mellitus following an accurate physical activity program at baseline, after 3 months and after 6 months. We found that there is a ameliorate of many anthropometric and biochemical parameters; furthermore, there is a statistical increase of orexin-A serum levels already after 3 months compared to baseline in T2DM subjects and also there is a strongly modulation in inflammatory cytokines expression. These found indicates that the physical activity has beneficial effects not only on anthropometric and biochemical parameters but also on orexin-A levels, and then on CNS.


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How to Cite

Polito, R., Francavilla, V. C., Ambrosi, A., Tartaglia, N., Tafuri, D., Monda, M., Messina, A., Sessa, F., Di Maio, G., Ametta, A., Scarinci, A., Monda, V., & Messina, G. (2020). The Orexin-A serum levels are strongly modulated by physical activity intervention in diabetes mellitus patients. Journal of Human Sport and Exercise, 15(2proc), S244-S251.

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